| Postmortem degradation of N-acetyl aspartate and N-acetyl aspartylglutamate: an HPLC analysis of different rat CNS regions. | |
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MedLine Citation:
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PMID: 11519729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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N-acetyl aspartate (NAA), a putative marker of neuronal injury, can be measured non-invasively in patients by magnetic resonance spectroscopy (MRS). Interpretation of in vivo MRS data, however, requires neuropathological correlates to NAA alterations using autopsy or biopsy material. Since detailed hydrolysis data is lacking, NAA and the related dipeptide N-acetyl aspartylglutamate (NAAG) were quantified by high performance liquid chromatography (HPLC) in different rat CNS regions over 24 h postmortem. Both molecules decreased rapidly 1-4 h postmortem, and subsequently slower with time. The average reduction at 24 h was 46% and 38% for NAA and NAAG respectively. The NAA reduction was proportionally smaller in cortical areas (34-37%) compared to more caudal regions (54-58%). An exception was the optic nerve, a pure white matter tract, where NAA and NAAG hydrolysis was slower. The NAA/NAAG ratio remained relatively constant, but exhibited marked regional differences. The data show a significant postmortem degradation of NAA and NAAG that needs to be considered when these compounds are studied ex-vivo. |
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Authors:
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J Battistuta; C Bjartmar; B D Trapp |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neurochemical research Volume: 26 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-08-24 Completed Date: 2002-01-29 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: United States |
Other Details:
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Languages: eng Pagination: 695-702 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Cleveland State University, Ohio, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aspartic Acid / analogs & derivatives*, metabolism* Central Nervous System / metabolism* Chromatography, High Pressure Liquid Dipeptides / metabolism* Hydrolysis Male Postmortem Changes* Rats Rats, Sprague-Dawley Tissue Distribution |
| Grant Support | |
ID/Acronym/Agency:
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NS35058/NS/NINDS NIH HHS; NS38667/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Dipeptides; 3106-85-2/N-acetyl-1-aspartylglutamic acid; 56-84-8/Aspartic Acid; 997-55-7/N-acetylaspartate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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