Document Detail

Postexercise skeletal muscle glucose transport is normal in kininogen-deficient rats.
MedLine Citation:
PMID:  21200341     Owner:  NLM     Status:  MEDLINE    
PURPOSE: This study aimed to determine whether the postexercise (PEX) increase in insulin-dependent or insulin-independent GT is reduced in rats deficient in plasma kininogen versus normal rats.
METHODS: Male Brown Norway (BN) and Brown Norway Katholiek (BNK; plasma kininogen-deficient) rats were studied. BN and BNK rats were assigned to exercise (4×30-min swim) or sedentary (SED) groups. Rats were anesthetized immediately (0hPEX) or 3 h (3hPEX) after exercise. For 0hPEX and 0hSED rats, one epitrochlearis muscle per rat was used for AMPK phosphorylation and muscle glycogen analyses. The contralateral muscle was incubated with [H]-3-O-methylglucose (3-MG) for GT assay. For 3hPEX and 3hSED rats, one muscle from each rat was incubated without insulin, and the contralateral muscle was incubated with 60 μU·mL insulin, and both muscles were incubated with 3-MG for GT measurement.
RESULTS: For 0hPEX versus 0hSED, both BN and BNK rats had greater insulin-independent GT and AMPK phosphorylation with reduced glycogen after exercise. No genotype effects were found 0hPEX. There was a significant main effect of exercise (3hPEX>3hSED) and no interaction between exercise and genotype for basal or insulin-stimulated GT.
CONCLUSIONS: Plasma kininogen deficiency did not alter insulin-independent GT, AMPK phosphorylation, or glycogen depletion 0hPEX or insulin-dependent GT 3hPEX, suggesting that normal plasma kininogen is not essential for these important exercise effects.
George G Schweitzer; Gregory D Cartee
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  43     ISSN:  1530-0315     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-22     Completed Date:  2011-10-28     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1148-53     Citation Subset:  IM; S    
Muscle Biology Laboratory, School of Kinesiology, Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA.
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MeSH Terms
3-O-Methylglucose / metabolism
Biological Transport / drug effects,  physiology
Glucose / metabolism*,  physiology
Glycogen / metabolism
Hypoglycemic Agents / pharmacology
Insulin / pharmacology
Kininogens / blood,  deficiency*
Muscle, Skeletal / metabolism*,  physiology
Physical Conditioning, Animal / physiology*
Grant Support
Reg. No./Substance:
0/Hypoglycemic Agents; 0/Insulin; 0/Kininogens; 146-72-5/3-O-Methylglucose; 50-99-7/Glucose; 9005-79-2/Glycogen

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