Document Detail

Post-translational and transcriptional regulation of glycolipid glycosyltransferase genes in apoptotic breast carcinoma cells: VII. Studied by DNA-microarray after treatment with L-PPMP.
MedLine Citation:
PMID:  19184418     Owner:  NLM     Status:  MEDLINE    
Functions of glycosphingolipids on the eukaryotic cell membranes during the onset of oncogenic processes and cell death are not well understood. Inhibitors of glycosphingolipid biosynthesis were recently found to trigger apoptosis in many carcinoma including breast cancer SKBR-3, MCF-7, and MDA-468 cells through either intrinsic or extrinsic apoptotic pathways as we previously reported. These anti-cancer inhibitors could increase ceramide concentration by blocking functions of glycolipid glycosyltransferases (GLTs). In this study, using a novel fluorescent dye (ASK-0) revealed the damage of cell organelle membranes by an inhibitor of glucosylceramide biosynthesis (L-PPMP). A highly drug- and cell-dependent regulation of MAPKs was also found by cis-platin and L-PPMP when inducing apoptosis in SKBR-3, MCF-7, and MDA-468 cells. A dose and time-dependent regulation of GLTs were investigated by enzymatic assay and DNA microarray analyses. These GLTs are involved in biosynthesis of Le(X) and sialosyl-Le(X) (neolactosyl-ceramide series) such as GalT-4 (UDP-Gal: LcOse3cer beta-galactosyltransferase, GalT-5 (UDP-Gal: nLcOse4Cer alpha1, 3galactosyltransferase, FucT-3 (GDP-Fucose: LM1 alpha1, 4fucosyltransferase). A similar effect was observed with the GLTs involved in the biosyntheses of Gg-series gangliosides, such as SAT-4 (CMP-NeuAc: GgOse4Cer alpha2, 3sialyltransferase, and SAT-2 (CMP-NeuAc: GM3 alpha2, 8sialyltransferase). The glycol-related gene DNA-microarrays also suggested the transcriptional regulation of several GLTs involved in the biosynthesis of neolactosylceramide containing cell-surface antigens in these apoptotic breast carcinoma cells. In the early apoptotic stages (2 to 6 h after L-PPMP treatment) in addition to GlcT-1 gene, several genes (betaGalTs and betaGlcNAcTs) in the SA-Le(a) pathway were stimulated.
Rui Ma; N Matthew Decker; Vesta Anilus; Joseph R Moskal; Joseph Burgdorf; James R Johnson; Manju Basu; Sipra Banerjee; Subhash Basu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-01-29
Journal Detail:
Title:  Glycoconjugate journal     Volume:  26     ISSN:  1573-4986     ISO Abbreviation:  Glycoconj. J.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-23     Completed Date:  2009-10-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  647-61     Citation Subset:  IM    
Department of Chemistry and Biochemistry, The University of Notre Dame, Notre Dame, IN 46556, USA.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Cell Line, Tumor
Cisplatin / pharmacology
Fucosyltransferases / genetics,  metabolism
Galactosyltransferases / genetics,  metabolism
Gene Expression Regulation, Enzymologic / drug effects*
Gene Expression Regulation, Neoplastic / drug effects
Glycosyltransferases / genetics*,  metabolism*
Models, Biological
Morpholines / pharmacology
Oligonucleotide Array Sequence Analysis
Grant Support
Reg. No./Substance:
0/Antineoplastic Agents; 0/Morpholines; 15663-27-1/Cisplatin; EC 2.4.-/Glycosyltransferases; EC 2.4.1.-/Fucosyltransferases; EC 2.4.1.-/Galactosyltransferases; EC 2.4.1.-/triaosylceramide 1,4-galactosyltransferase; EC 4-alpha-L-fucosyltransferase

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