Document Detail

Post-transcriptional control of Na,K-ATPase activity and cell growth by a splice variant of FXYD2 protein with modified mRNA.
MedLine Citation:
PMID:  21460224     Owner:  NLM     Status:  MEDLINE    
In kidney, FXYD proteins regulate Na,K-ATPase in a nephron segment-specific way. FXYD2 is the most abundant renal FXYD but is not expressed in most renal cell lines unless induced by hypertonicity. Expression by transfection of FXYD2a or FXYD2b splice variants in NRK-52E cells reduces the apparent Na(+) affinity of the Na,K-ATPase and slows the cell proliferation rate. Based on RT-PCR, mRNAs for both splice variants were expressed in wild type NRK-52E cells as low abundance species. DNA sequencing of the PCR products revealed a base alteration from C to T in FXYD2b but not FXYD2a from both untreated and hypertonicity-treated NRK-52E cells. The 172C→T sequence change exposed a cryptic KKXX endoplasmic reticulum retrieval signal via a premature stop codon. The truncation affected trafficking of FXYD2b and its association with Na,K-ATPase and blocked its effect on enzyme kinetics and cell growth. The data may be explained by altered splicing or selective RNA editing of FXYD2b, a supplementary process that would ensure that it was inactive even if transcribed and translated, in these cells that normally express only FXYD2a. 172C→T mutation was also identified after mutagenesis of FXYD2b by error-prone PCR coupled with a selection for cell proliferation. Furthermore, the error-prone PCR alone introduced the mutation with high frequency, implying a structural peculiarity. The data confirm truncation of FXYD2b as a potential mechanism to regulate the amount of FXYD2 at the cell surface to control activity of Na,K-ATPase and cell growth.
Kathleen J Sweadner; Jennifer L Pascoa; Cynthia A Salazar; Elena Arystarkhova
Related Documents :
25193434 - Differential effects of triclosan on the activation of mouse and human peroxisome proli...
25303534 - Interplay of cell shape and division orientation promotes robust morphogenesis of devel...
25479044 - Identification of amacrine subtypes that express the atypical cadherin celsr3.
25333004 - Cell sheets engineering for esophageal regenerative medicine.
1391054 - Achievement of complete bacillus subtilis microcycle sporulation by the addition of s-a...
20690004 - Intestinal mesenchymal cells.
Publication Detail:
Type:  Journal Article; Research Support, American Recovery and Reinvestment Act; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-04-01
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  286     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-16     Completed Date:  2011-07-26     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  18290-300     Citation Subset:  IM    
Copyright Information:
© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
Laboratory of Membrane Biology, Department of Neurosurgery, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line, Tumor
Endoplasmic Reticulum / genetics,  metabolism*
Isoenzymes / biosynthesis,  genetics
Protein Sorting Signals / physiology*
RNA Editing / physiology*
RNA, Messenger / biosynthesis*,  genetics
Sodium-Potassium-Exchanging ATPase / biosynthesis*,  genetics
Grant Support
Reg. No./Substance:
0/Isoenzymes; 0/Protein Sorting Signals; 0/RNA, Messenger; EC 3.6.1.-/Fxyd2 protein, rat; EC ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Age-dependent accumulation of soluble amyloid beta (Abeta) oligomers reverses the neuroprotective ef...
Next Document:  Evidence that the histone methyltransferase Dot1 mediates global genomic repair by methylating histo...