| Post-prostatic massage fluid/urine as an alternative to semen for studying male genitourinary HIV-1 shedding. | |
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MedLine Citation:
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PMID: 21278399 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Genitourinary tract samples are required to investigate male HIV-1 infectivity. Because semen collection is often impractical, the acceptability, feasibility and validity of post-prostatic massage fluid/urine (post-PMF/U) was evaluated for studying male genitourinary HIV-1 shedding. METHODS: HIV-1-seropositive men were evaluated after 48 h of sexual abstinence. At each visit, a clinician performed prostatic massage, then post-PMF/U and blood were collected. Participants provided semen specimens 1 week later. An audio computer-assisted self-interview (ACASI) administered after each specimen collection evaluated acceptability, adherence to instructions and recent genitourinary symptoms. HIV-1 RNA was quantified using a real-time PCR assay. Detection and quantitation of HIV-1 RNA and stability over visits were compared for semen, post-PMF/U and blood. RESULTS: Post-PMF/U was successfully obtained at 106 visits (64%) and semen at 136 visits (81%, p<0.001). In ACASI, discomfort was rated higher for post-PMF/U collection (p=0.003), but there was no significant difference in acceptability. Detection of HIV-1 RNA in post-PMF/U was associated with detection in semen (p=0.02). Semen and post-PMF/U HIV-1-RNA levels were correlated (ρ=0.657, p<0.001). Concordance of results at repeat visits was 78.9% for post-PMF/U (κ=0.519, p=0.02) and 89.5% for both blood and semen (κ=0.774, p=0.001). CONCLUSIONS: Although semen collections were more successful, both post-PMF/U and semen collections were acceptable to many participants. HIV-1 RNA detection and levels were closely associated in semen and post-PMF/U, and results were relatively stable across visits. To assess male HIV-1 infectivity, post-PMF/U may represent a valid alternative when semen cannot be obtained. |
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Authors:
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Susan M Graham; John N Krieger; Peter L M Githua; Lorraine W Wamuyu; Steven Wale; Kelly M Ramko; Joan A Dragavon; Charles H Muller; Sarah E Holte; Kishor N Mandaliya; R Scott McClelland; Norbert M Peshu; Eduard J Sanders; Robert W Coombs |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural Date: 2011-01-29 |
Journal Detail:
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Title: Sexually transmitted infections Volume: 87 ISSN: 1472-3263 ISO Abbreviation: Sex Transm Infect Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-22 Completed Date: 2011-08-08 Revised Date: 2012-04-04 |
Medline Journal Info:
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Nlm Unique ID: 9805554 Medline TA: Sex Transm Infect Country: England |
Other Details:
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Languages: eng Pagination: 232-7 Citation Subset: IM |
Affiliation:
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University of Washington, Box 359909, 325 Ninth Avenue, Seattle, WA 98104-2499, USA. grahamsm@u.washington.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bodily Secretions Feasibility Studies HIV Infections / virology* HIV-1* Humans Male Massage* Middle Aged Prognosis Prostate RNA, Viral / analysis Semen / virology* Urogenital System / virology* Virus Shedding* |
| Grant Support | |
ID/Acronym/Agency:
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K23 AI069990-04/AI/NIAID NIH HHS; K23 AI69990/AI/NIAID NIH HHS; P30-AI027757/AI/NIAID NIH HHS; R21 HD055864/HD/NICHD NIH HHS; R21 HD055864-02/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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