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Post-fermentative production of glutathione by baker's yeast (S. cerevisiae) in compressed and dried forms.
MedLine Citation:
PMID:  22705095     Owner:  NLM     Status:  Publisher    
The study was aimed at investigating the best biotransformation conditions to increase intracellular glutathione (GSH) levels in samples of baker's yeast (S. cerevisiae) employing either the commercially available compressed and dried forms. Glucose, GSH precursors amino acids, as well as other cofactors, were dissolved in a biotransformation solution and yeast cells were added (5%dcw). Two response surface central composite designs (RSCCD) were performed in sequence: in the first step the influence of amino acid composition (cysteine, glycine, glutamic acid and serine) on GSH accumulation was investigated; once setup their formulation, the influence of other components was studied. Initial GSH content was found 0.53 and 0.47%dcw for compressed and dried forms. GSH accumulation ability of baker's yeast in compressed form was higher at the beginning of shelf life, i.e. in the first week, and a maximum of 2.04%dcw was obtained. Performance of yeast in dried form was not found satisfactory, as the maximum GSH level was 1.18%dcw. When cysteine lacks from the reaction solution, yeast cells do not accumulate GSH. With dried yeast, the highest GSH yields occurred when cysteine was set at 3g/L, glycine and glutamic acid at least at 4g/L, without serine. Employing compressed yeast, the highest GSH yields occurred when cysteine and glutamic acid were set at 2-3g/L, while glycine and serine higher than 2g/L. Results allowed to setup an optimal and feasible procedure to obtain GSH-enriched yeast biomass, with up to 3-fold increase respect to initial content.
Alida Musatti; Matilde Manzoni; Manuela Rollini
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-12
Journal Detail:
Title:  New biotechnology     Volume:  -     ISSN:  1876-4347     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101465345     Medline TA:  N Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
DiSTAM, Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche. Università degli Studi di Milano, Via G. Celoria 2, 20133 Milano, Italy.
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