Document Detail


Post-exercise carbohydrate plus whey protein hydrolysates supplementation increases skeletal muscle glycogen level in rats.
MedLine Citation:
PMID:  19593593     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies showed that a combination of carbohydrate and protein was more effective than carbohydrate alone for replenishing muscle glycogen after exercise. However, it remains to be unclear whether the source or degree of hydrolysis of dietary protein influences post-exercise glycogen accumulation. The aim of this study was to compare the effect of dietary protein type on glycogen levels in the post-exercise phase, and to investigate the effects of post-exercise carbohydrate and protein supplementation on phosphorylated enzymes of Akt/PKB and atypical PKCs. Male Sprague-Dawley rats, trained for 3 days, swam with a 2% load of body weight for 4 h to deplete skeletal muscle glycogen. Immediately after the glycogen-depleting exercise, one group was killed, whereas the other groups were given either glucose or glucose plus protein (whey protein, whey protein hydrolysates (WPH), casein hydrolysates or branched-chain amino acid (BCAA) solutions. After 2 h, the rats were killed, and the triceps muscles quickly excised. WPH caused significant increases in skeletal muscle glycogen level (5.01 +/- 0.24 mg/g), compared with whey protein (4.23 +/- 0.24 mg/g), BCAA (3.92 +/- 0.18 mg/g) or casein hydrolysates (2.73 +/- 0.22 mg/g). Post-exercise ingestion of glucose plus WPH significantly increased both phosphorylated Akt/PKB (131%) and phosphorylated PKCzeta (154%) levels compared with glucose only. There was a significant positive correlation between skeletal muscle glycogen content and phosphorylated Akt/PKB (r = 0.674, P < 0.001) and PKCzeta (r = 0.481, P = 0.017). Post-exercise supplementation with carbohydrate and WPH increases skeletal muscle glycogen recovery by activating key enzymes such as Akt/PKB and atypical PKCs.
Authors:
Masashi Morifuji; Atsushi Kanda; Jinichiro Koga; Kentaro Kawanaka; Mitsuru Higuchi
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2009-07-11
Journal Detail:
Title:  Amino acids     Volume:  38     ISSN:  1438-2199     ISO Abbreviation:  Amino Acids     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-07     Completed Date:  2010-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200312     Medline TA:  Amino Acids     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  1109-15     Citation Subset:  IM    
Affiliation:
Food and Health R&D Laboratories, Meiji Seika Kaisha Ltd., 5-3-1 Chiyoda, Sakado, Saitama, 350-0289, Japan. masashi_morifuji@fuji.waseda.jp
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Amino Acids, Branched-Chain / administration & dosage,  analysis
Animals
Antibodies, Phospho-Specific
Caseins / administration & dosage,  chemistry
Dietary Carbohydrates / administration & dosage*
Dietary Supplements* / analysis
Enzyme Activation
Glycogen / metabolism*
Male
Milk Proteins / administration & dosage*,  chemistry
Muscle, Skeletal / metabolism*
Phosphorylation
Physical Exertion*
Protein Hydrolysates / administration & dosage*,  chemistry
Protein Kinase C / metabolism
Proto-Oncogene Proteins c-akt / metabolism
Rats
Rats, Sprague-Dawley
Swimming
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Amino Acids, Branched-Chain; 0/Antibodies, Phospho-Specific; 0/Caseins; 0/Dietary Carbohydrates; 0/Milk Proteins; 0/Protein Hydrolysates; 0/whey protein; 65072-00-6/casein hydrolysate; 9005-79-2/Glycogen; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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