| Post-exercise carbohydrate plus whey protein hydrolysates supplementation increases skeletal muscle glycogen level in rats. | |
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MedLine Citation:
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PMID: 19593593 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent studies showed that a combination of carbohydrate and protein was more effective than carbohydrate alone for replenishing muscle glycogen after exercise. However, it remains to be unclear whether the source or degree of hydrolysis of dietary protein influences post-exercise glycogen accumulation. The aim of this study was to compare the effect of dietary protein type on glycogen levels in the post-exercise phase, and to investigate the effects of post-exercise carbohydrate and protein supplementation on phosphorylated enzymes of Akt/PKB and atypical PKCs. Male Sprague-Dawley rats, trained for 3 days, swam with a 2% load of body weight for 4 h to deplete skeletal muscle glycogen. Immediately after the glycogen-depleting exercise, one group was killed, whereas the other groups were given either glucose or glucose plus protein (whey protein, whey protein hydrolysates (WPH), casein hydrolysates or branched-chain amino acid (BCAA) solutions. After 2 h, the rats were killed, and the triceps muscles quickly excised. WPH caused significant increases in skeletal muscle glycogen level (5.01 +/- 0.24 mg/g), compared with whey protein (4.23 +/- 0.24 mg/g), BCAA (3.92 +/- 0.18 mg/g) or casein hydrolysates (2.73 +/- 0.22 mg/g). Post-exercise ingestion of glucose plus WPH significantly increased both phosphorylated Akt/PKB (131%) and phosphorylated PKCzeta (154%) levels compared with glucose only. There was a significant positive correlation between skeletal muscle glycogen content and phosphorylated Akt/PKB (r = 0.674, P < 0.001) and PKCzeta (r = 0.481, P = 0.017). Post-exercise supplementation with carbohydrate and WPH increases skeletal muscle glycogen recovery by activating key enzymes such as Akt/PKB and atypical PKCs. |
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Authors:
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Masashi Morifuji; Atsushi Kanda; Jinichiro Koga; Kentaro Kawanaka; Mitsuru Higuchi |
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Publication Detail:
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Type: Comparative Study; Journal Article Date: 2009-07-11 |
Journal Detail:
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Title: Amino acids Volume: 38 ISSN: 1438-2199 ISO Abbreviation: Amino Acids Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-07 Completed Date: 2010-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9200312 Medline TA: Amino Acids Country: Austria |
Other Details:
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Languages: eng Pagination: 1109-15 Citation Subset: IM |
Affiliation:
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Food and Health R&D Laboratories, Meiji Seika Kaisha Ltd., 5-3-1 Chiyoda, Sakado, Saitama, 350-0289, Japan. masashi_morifuji@fuji.waseda.jp |
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Amino Acids, Branched-Chain / administration & dosage, analysis Animals Antibodies, Phospho-Specific Caseins / administration & dosage, chemistry Dietary Carbohydrates / administration & dosage* Dietary Supplements* / analysis Enzyme Activation Glycogen / metabolism* Male Milk Proteins / administration & dosage*, chemistry Muscle, Skeletal / metabolism* Phosphorylation Physical Exertion* Protein Hydrolysates / administration & dosage*, chemistry Protein Kinase C / metabolism Proto-Oncogene Proteins c-akt / metabolism Rats Rats, Sprague-Dawley Swimming |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Amino Acids, Branched-Chain; 0/Antibodies, Phospho-Specific; 0/Caseins; 0/Dietary Carbohydrates; 0/Milk Proteins; 0/Protein Hydrolysates; 0/whey protein; 65072-00-6/casein hydrolysate; 9005-79-2/Glycogen; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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