Document Detail

Possible toxic effects of normal and diabetic patient serum on pancreatic B-cells.
MedLine Citation:
PMID:  342324     Owner:  NLM     Status:  MEDLINE    
Serum from normal blood-donors and juvenile diabetic patients inhibited Rb+ accumulation and stimulated release of 51Cr and insulin in suspensions of dispersed pancreatic islet cells prepared from ob/ob mouse islets, which are rich in B-cells. The effects indicate the presence of a B-cytotoxic factor in human serum. Serum from mouse and fetal calf also inhibited the islet cell accumulation of Rb+. Toxicity was not suppressed by treating serum with protein A-Sepharose and did not correlate with islet cell binding of fluorescent antibodies to human immunoglobulin. Whereas all sera inhibited Rb+ accumulation, 3 of 6 diabetic patient sera, but no blood-donor serum, made the cells fluoresce on exposure to the fluorescent antibodies. Supporting a dependence on complement, toxicity remained after dialysis, but was destroyed by treating serum with zymosan-A or heating at 56 degrees for 30 min.
A Lernmark; J Sehlin; I B Täljedal; H Kromann; J Nerup
Related Documents :
14727874 - Improved function of rat islets upon co-microencapsulation with sertoli's cells in algi...
7326214 - Involution of the pancreas after ligation of the pancreatic ducts. i: a histological st...
10880374 - The role of intracellular calcium signaling in premature protease activation and the on...
10458104 - Histopathological and immunohistochemical analysis of the endocrine and exocrine pancre...
2164484 - Fusion-mediated microinjection of liposome-enclosed dna into cultured cells with the ai...
23569434 - Wnt5a inhibits the proliferation and melanogenesis of melanocytes.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diabetologia     Volume:  14     ISSN:  0012-186X     ISO Abbreviation:  Diabetologia     Publication Date:  1978 Jan 
Date Detail:
Created Date:  1978-04-26     Completed Date:  1978-04-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  25-31     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
B-Lymphocytes / immunology*
Chromium Radioisotopes
Cytotoxicity, Immunologic
Diabetes Mellitus, Type 1 / blood*
Fluorescent Antibody Technique
Guinea Pigs
Insulin / metabolism
Islets of Langerhans / immunology*,  metabolism
Reg. No./Substance:
0/Antibodies; 0/Chromium Radioisotopes; 0/Radioisotopes; 11061-68-0/Insulin; 7440-17-7/Rubidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Extrapancreatic glucagons.
Next Document:  A comparison between chromic catgut and polyglycolic acid sutures in episiotomy repair (author's tra...