Document Detail


Possible toxic effects of normal and diabetic patient serum on pancreatic B-cells.
MedLine Citation:
PMID:  342324     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Serum from normal blood-donors and juvenile diabetic patients inhibited Rb+ accumulation and stimulated release of 51Cr and insulin in suspensions of dispersed pancreatic islet cells prepared from ob/ob mouse islets, which are rich in B-cells. The effects indicate the presence of a B-cytotoxic factor in human serum. Serum from mouse and fetal calf also inhibited the islet cell accumulation of Rb+. Toxicity was not suppressed by treating serum with protein A-Sepharose and did not correlate with islet cell binding of fluorescent antibodies to human immunoglobulin. Whereas all sera inhibited Rb+ accumulation, 3 of 6 diabetic patient sera, but no blood-donor serum, made the cells fluoresce on exposure to the fluorescent antibodies. Supporting a dependence on complement, toxicity remained after dialysis, but was destroyed by treating serum with zymosan-A or heating at 56 degrees for 30 min.
Authors:
A Lernmark; J Sehlin; I B Täljedal; H Kromann; J Nerup
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diabetologia     Volume:  14     ISSN:  0012-186X     ISO Abbreviation:  Diabetologia     Publication Date:  1978 Jan 
Date Detail:
Created Date:  1978-04-26     Completed Date:  1978-04-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  25-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies
B-Lymphocytes / immunology*
Chromium Radioisotopes
Cytotoxicity, Immunologic
Diabetes Mellitus, Type 1 / blood*
Fluorescent Antibody Technique
Guinea Pigs
Humans
Insulin / metabolism
Islets of Langerhans / immunology*,  metabolism
Mice
Radioisotopes
Rubidium
Chemical
Reg. No./Substance:
0/Antibodies; 0/Chromium Radioisotopes; 0/Radioisotopes; 11061-68-0/Insulin; 7440-17-7/Rubidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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