Document Detail

Possible role of sialocompounds in the uptake of choline into synaptosomes and nerve cell cultures.
MedLine Citation:
PMID:  7110505     Owner:  NLM     Status:  MEDLINE    
Incubation of primary nerve cell cultures and of crude synaptosomal preparations with neuraminidase released sialic acid from both gangliosides and sialoglycoproteins. After this treatment, the pattern of ganglioside distribution was severely modified with a decrease of polysialogangliosides (GD1b, GT1b, Gt1L, GQ1) and a dramatic increase in monosialoganglioside GM1. The choline influx into neuraminidase treated cells and organelles was reduced by 30--50% but the efflux was unmodified. In particular the high affinity mechanism of choline uptake disappeared and the low affinity mechanism was modified in both cases. The disappearance of the high affinity uptake mechanism was not followed by a decreased acetylcholine synthesis as it should be if the current theories on choline uptake and acetylcholine synthesis are correct. Our present data thus confirm our previous hypothesis that choline metabolism regulates choline uptake rather than the other way round as is suggested by the theories most widely accepted at present. Choline uptake was unaffected by pretreatment of cells and organelles with tetanus toxin suggesting that the effect of neuraminidase on the choline uptake were either mediated through glycoproteins or through gangliosides other than those which bind to tetanus toxin (GD1b and GT1b). Several speculative models for explaining the effect of neuraminidase on choline uptake are proposed.
R Massarelli; T Y Wong; S Harth; J C Louis; L Freysz; H Dreyfus
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemical research     Volume:  7     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1982 Mar 
Date Detail:
Created Date:  1982-10-21     Completed Date:  1982-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  301-16     Citation Subset:  IM    
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MeSH Terms
Brain / cytology
Cell Line
Chick Embryo
Choline / metabolism*
Gangliosides / physiology
Glycoproteins / physiology
Neuraminidase / pharmacology
Neurons / drug effects,  metabolism*
Sialic Acids / physiology*
Synaptosomes / metabolism*
Reg. No./Substance:
0/Gangliosides; 0/Glycoproteins; 0/Sialic Acids; 62-49-7/Choline; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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