Document Detail

Possible role of fatty acids in milk as the regulator of the expression of cytosolic binding proteins for fatty acids and vitamin A through PPARalpha in developing rats.
MedLine Citation:
PMID:  18202540     Owner:  NLM     Status:  MEDLINE    
Fatty acids in milk are thought to play an important role in intestinal maturation and gene expression in the postnatal small intestine. In this study, we determined the jejunal mRNA levels, in rats, of peroxisome proliferator-activated receptor alpha (PPARalpha) and PPARdelta which are nuclear receptors for fatty acids. We also measured expression of their target genes during the postnatal period, namely liver type fatty acid-binding protein (L-FABP) and cellular retinol-binding protein, type II (CRBPII). The mRNA levels of PPARalpha, L-FABP and CRBPII, but not PPARdelta, gradually increased during the suckling period and then sharply declined to a low level at the end of the weaning period. Rat pups at 17 d of age, weaned to a high-fat diet, showed significantly greater mRNA levels of PPARalpha, L-FABP and CRBPII than those weaned to a low-fat diet. Oral administration of PPARalpha ligand, WY14,643 during four consecutive days of the weanling period caused a parallel increase in the mRNA levels of PPARalpha, L-FABP and CRBPII genes. Furthermore, caprylic acid and oleic acid, which are major components of fatty acids in milk, induced jejunal PPARalpha, L-FABP and CRBPII gene expression. Our results suggest that fatty acids in milk may play a pivotal role in maintaining an enhanced level of expression of L-FABP and CRBPII genes in the small intestine, presumably by acting as inducers of PPARalpha gene expression.
Kazuki Mochizuki; Hiroko Mochizuki; Hiroko Kawai; Yuko Ogura; Masaya Shimada; Sachiko Takase; Toshinao Goda
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of nutritional science and vitaminology     Volume:  53     ISSN:  0301-4800     ISO Abbreviation:  J. Nutr. Sci. Vitaminol.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2008-01-18     Completed Date:  2008-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0402640     Medline TA:  J Nutr Sci Vitaminol (Tokyo)     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  515-21     Citation Subset:  IM    
School of Food and Nutritional Sciences, The University of Shizuoka, Shizuoka, Japan.
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MeSH Terms
Animals, Suckling
Cytosol / metabolism
Fatty Acid-Binding Proteins / genetics,  metabolism*
Fatty Acids / metabolism*,  pharmacology
Gene Expression / drug effects,  physiology
Jejunum / physiology
Milk / metabolism*
PPAR alpha / genetics,  metabolism*
PPAR delta / genetics,  metabolism
RNA, Messenger / metabolism
Rats, Sprague-Dawley
Retinol-Binding Proteins, Cellular / genetics,  metabolism
Signal Transduction / drug effects,  physiology
Vitamin A / metabolism*
Vitamins / metabolism*
Reg. No./Substance:
0/Fatty Acid-Binding Proteins; 0/Fatty Acids; 0/PPAR alpha; 0/PPAR delta; 0/RNA, Messenger; 0/Retinol-Binding Proteins, Cellular; 0/Vitamins; 11103-57-4/Vitamin A

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