Document Detail


Possible release of nitric oxide from cholinergic axons in the thalamus by stimulation of the rat laterodorsal tegmental nucleus as measured with voltammetry.
MedLine Citation:
PMID:  8811424     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
By means of the differential direct current voltammetry technique with carbon fiber electrodes in urethane-anesthetized rats, we monitored nitric oxide (NO) concentrations in the thalamus in the basal condition and following electrical stimulation of the laterodorsal tegmental nucleus (LDT), whose neurons have the strongest activity of NADPH-diaphorase, or NO synthase, together with acetylcholine. NO levels, measured as the height of the peak at +970-1000 mV in the voltammetry (NO was soon oxidized in vivo to be detected at the voltage of this peak, so that NO levels in this report are, in the strict sense, levels of the oxidized metabolites reflecting very possibly those of NO in physiological conditions; see Section 2, Methods), increased just after repetitive stimulation of the LDT. Stimulation of the surrounding areas or the cerebellum produced virtually no change in NO levels. An intravenous injection of L-nitroarginine methyl ester reduced the basal level of NO, but stimulation of the LDT still increased NO levels, which may be due to very strong activity of NO synthase in the LDT neurons. These results are consistent with the notion that NO can be released from axons of the LDT neurons by their excitation.
Authors:
M Miyazaki; Y Kayama; T Kihara; K Kawasaki; E Yamaguchi; Y Wada; M Ikeda
Related Documents :
12033734 - Nadph-diaphorase distribution in the rabbit superior colliculus and co-localization wit...
11162644 - Modulation of neuronal nitric oxide release by soluble guanylyl cyclase in guinea pig c...
9502244 - Neurochemical organization of the macaque striate cortex: correlation of cytochrome oxi...
1717154 - Neurofilament protein triplet immunoreactivity in the dorsal root ganglia of the guinea...
2918994 - An in vitro preparation for studying motor pattern generation in the cerebellorubrospin...
9578434 - The search for cell assemblies in the working brain.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chemical neuroanatomy     Volume:  10     ISSN:  0891-0618     ISO Abbreviation:  J. Chem. Neuroanat.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-11-27     Completed Date:  1996-11-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8902615     Medline TA:  J Chem Neuroanat     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  203-7     Citation Subset:  IM    
Affiliation:
Department of Physiology, Fukushima Medical College, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cerebrovascular Circulation / physiology
Cholinergic Fibers / enzymology*
Electric Stimulation
Enzyme Inhibitors / pharmacology
Geniculate Bodies / blood supply,  cytology*,  metabolism
Male
Microelectrodes
NADPH Dehydrogenase / metabolism
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide / metabolism*
Nitric Oxide Synthase / metabolism
Pons / physiology*
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 10102-43-9/Nitric Oxide; 50903-99-6/NG-Nitroarginine Methyl Ester; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.6.99.1/NADPH Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Regional specific effects of nitric oxide donors and cGMP on the electrical activity of neurons in t...
Next Document:  3-Nitropropionic acid produces striatum selective lesions accompanied by iNOS expression.