Document Detail

Possible regulation of Oct60 transcription by a positive feedback loop in Xenopus oocytes.
MedLine Citation:
PMID:  23186935     Owner:  NLM     Status:  Publisher    
Summary The POU family subclass V (POU-V) proteins have important roles in maintaining cells in an undifferentiated state. In Xenopus, expression of the POU-V protein Oct60 was detected in oocytes and was found to decrease in blastula- to gastrula-stage embryos. In addition, Oct60 overexpression inhibits some signals in early embryogenesis, including Activin/Nodal, BMP, and Wnt signalling. In this report, we analysed mechanisms of Oct60 promoter activation and discovered that Oct60 transcription was activated ectopically in somatic nuclei by oocyte extract treatment. Promoter assays demonstrated that Oct60 transcription was activated in oocytes specifically and that this activation was dependent on an Octamer-Sox binding motif. ChIP assays showed that the Oct60 protein binds the motif. These results suggest that Oct60 transcription is regulated by a positive-feedback loop in Xenopus oocytes.
Keisuke Morichika; Makoto Sugimoto; Katsuki Yasuda; Tsutomu Kinoshita
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-28
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  -     ISSN:  1469-8730     ISO Abbreviation:  Zygote     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-9     Citation Subset:  -    
Department of Life Science, School of Science, Rikkyo University, 3-34-1 Nishi-ikebukuro, Toshima, Tokyo 178-8501, Japan.
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