| Possible key residues that determine left gastric artery blood flow response to PACAP in dogs. | |
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MedLine Citation:
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PMID: 20939117 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To determine the effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on left gastric artery (LGA) flow and to unveil the structural or functional important sites that may be critical for discrimination of different receptor subtypes. METHODS: Peptides, including PACAP-27, PACAP-38, amino acid substituted PACAP-27 and C-terminus truncated analogues PACAP (27-38), were synthesized by a simultaneous multiple solid-phase peptide synthesizer. Flow probes of an ultrasound transit-time blood flowmeter were placed around the LGA of beagle dogs. When peptides were infused intravenously, the blood flow was measured. RESULTS: [Ala4, Val5]-PACAP-27 caused a concentration-dependent vasodepressor action which was similar to that caused by PACAP-27. The LGA blood flow response to [Ala4, Val5]-PACAP-27 was significantly higher than that to PACAP-27, which was similar to that to vasoactive intestinal polypeptide (VIP) at the same dose. [Ala6]-PACAP-27 did not increase the peak LGA flow. [Gly8]-PACAP-27 showed a similar activity to VIP. [Asn24, Ser25, Ile26]-PACAP-27 did not change the activity of peptides at all doses. CONCLUSION: NH2 terminus is more important to biological activity of peptides and specific receptor recognition than COOH-terminus. |
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Authors:
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Mu-Xin Wei; Ping Hu; Ping Wang; Satoru Naruse; Kiyoshi Nokihara; Victor Wray; Tsuyoshi Ozaki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 16 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-12 Completed Date: 2011-01-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 4865-70 Citation Subset: IM |
Affiliation:
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Department of Traditional Chinese Medicine, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. weimuxin@njmu.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Arteries / drug effects* Dogs Hemodynamics / drug effects Molecular Sequence Data Peptides / chemical synthesis, genetics, pharmacology Pituitary Adenylate Cyclase-Activating Polypeptide / genetics*, pharmacology* Regional Blood Flow / drug effects* Stomach / blood supply* Vasoactive Intestinal Peptide / genetics, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Peptides; 0/Pituitary Adenylate Cyclase-Activating Polypeptide; 37221-79-7/Vasoactive Intestinal Peptide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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