Document Detail


Possible key residues that determine left gastric artery blood flow response to PACAP in dogs.
MedLine Citation:
PMID:  20939117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To determine the effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on left gastric artery (LGA) flow and to unveil the structural or functional important sites that may be critical for discrimination of different receptor subtypes.
METHODS: Peptides, including PACAP-27, PACAP-38, amino acid substituted PACAP-27 and C-terminus truncated analogues PACAP (27-38), were synthesized by a simultaneous multiple solid-phase peptide synthesizer. Flow probes of an ultrasound transit-time blood flowmeter were placed around the LGA of beagle dogs. When peptides were infused intravenously, the blood flow was measured.
RESULTS: [Ala4, Val5]-PACAP-27 caused a concentration-dependent vasodepressor action which was similar to that caused by PACAP-27. The LGA blood flow response to [Ala4, Val5]-PACAP-27 was significantly higher than that to PACAP-27, which was similar to that to vasoactive intestinal polypeptide (VIP) at the same dose. [Ala6]-PACAP-27 did not increase the peak LGA flow. [Gly8]-PACAP-27 showed a similar activity to VIP. [Asn24, Ser25, Ile26]-PACAP-27 did not change the activity of peptides at all doses.
CONCLUSION: NH2 terminus is more important to biological activity of peptides and specific receptor recognition than COOH-terminus.
Authors:
Mu-Xin Wei; Ping Hu; Ping Wang; Satoru Naruse; Kiyoshi Nokihara; Victor Wray; Tsuyoshi Ozaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  16     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-12     Completed Date:  2011-01-28     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  4865-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Arteries / drug effects*
Dogs
Hemodynamics / drug effects
Molecular Sequence Data
Peptides / chemical synthesis,  genetics,  pharmacology
Pituitary Adenylate Cyclase-Activating Polypeptide / genetics*,  pharmacology*
Regional Blood Flow / drug effects*
Stomach / blood supply*
Vasoactive Intestinal Peptide / genetics,  pharmacology
Chemical
Reg. No./Substance:
0/Peptides; 0/Pituitary Adenylate Cyclase-Activating Polypeptide; 37221-79-7/Vasoactive Intestinal Peptide
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