| Possible involvement of nitric oxide in carbachol-induced activation of transglutaminase in rat superior cervical sympathetic ganglia. | |
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MedLine Citation:
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PMID: 7753507 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The addition of a muscarinic agonist, carbachol (Carb, 0.1 mM), to a physiological medium markedly increased Ca(2+)-dependent transglutaminase (TG) activity (approximately 10-fold) in isolated rat superior cervical sympathetic ganglia (SCG) following in vitro aerobic incubation for 30 min at 37 degrees C. The Carb-evoked stimulation of ganglionic TG activity was considerably reduced (-51%) in the presence of NG-monomethyl-L-arginine (L-NMMA, 50 microM), a selective inhibitor of nitric oxide (NO) synthase. While the suppressant effect of L-NMMA was completely eliminated by the addition of an excess concentration of L-arginine (0.5 mM), a precursor of NO. These observations imply that Carb-induced TG activation possibly involves NO mediation in SCG tissue. The Carb-induced elevation in ganglionic TG activity was markedly reduced (-84%) at as early as 15 min of incubation in the medium containing hemoglobin (Hb) (20 microM), an agent that scavenges only extracellular NO gas. Thus, it is evident that a large fraction of NO released from inside the neuronal cells to extracellular space could rapidly diffuse back into the same group of cells to induce activation of the tissue TG. Methylene blue (MB), an inhibitor of soluble guanylate cyclase (GC), at 0.5 mM, a concentration which is effective in almost abolishing the Carb-evoked synthesis of cyclic GMP (cGMP), had no effect on ganglionic TG activation induced by Carb. Therefore, an increase in cGMP synthesis mediated by NO might not participate in NO-dependent ganglionic TG activation following the stimulation with Carb.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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M Ando; T Tatematsu; S Kusudo; K Fujita; Y Nagata |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuroscience research Volume: 21 ISSN: 0168-0102 ISO Abbreviation: Neurosci. Res. Publication Date: 1995 Jan |
Date Detail:
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Created Date: 1995-06-22 Completed Date: 1995-06-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8500749 Medline TA: Neurosci Res Country: IRELAND |
Other Details:
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Languages: eng Pagination: 267-72 Citation Subset: IM |
Affiliation:
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Department of Physiology, Fujita Health University School of Medicine, Aichi, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine / analogs & derivatives, pharmacology Calcium / physiology Carbachol / antagonists & inhibitors, pharmacology* Cyclic GMP / biosynthesis Enzyme Activation / drug effects Female Hemoglobins / pharmacology Male Methylene Blue / pharmacology Nitric Oxide / antagonists & inhibitors, physiology* Rats Rats, Wistar Superior Cervical Ganglion / drug effects, enzymology* Transglutaminases / metabolism* omega-N-Methylarginine |
| Chemical | |
Reg. No./Substance:
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0/Hemoglobins; 10102-43-9/Nitric Oxide; 17035-90-4/omega-N-Methylarginine; 51-83-2/Carbachol; 61-73-4/Methylene Blue; 74-79-3/Arginine; 7440-70-2/Calcium; 7665-99-8/Cyclic GMP; EC 2.3.2.13/Transglutaminases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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