Document Detail

Possible involvement of microfilaments in the regulation of Sertoli cell aromatase activity.
MedLine Citation:
PMID:  7589787     Owner:  NLM     Status:  MEDLINE    
Recent observations indicate that Sertoli cell aromatase activity decreases when cultures are performed at high density. Increasing cell density modifies cell shape in culture from flat cells with visible anchorage sites and abundant intercellular spaces to cells with higher profiles that form a uniform epithelial sheet with no intercellular spaces. Changes in cell architecture are associated with reorganization of the cytoskeleton components. In this report, we have tested whether disruption of microfilaments and microtubules by cytochalasin B and colchicine, respectively, has any effect on the ability of FSH to stimulate aromatase activity. Cytochalasin B, but not colchicine, significantly enhanced aromatase activity in FSH and dbcAMP stimulated cells. The increase in aromatase activity was accompanied by a striking change in cell morphology. Time course studies suggested that microfilament organization is involved in some metabolic event which occurs sometime between 2 and 4 h after the initial steps of FSH action. The reversibility of the biochemical and morphological changes induced by cytochalasin B was demonstrated. The effect of cytochalasin B was observed in high but not in low-density cultures, suggesting that microfilament organization in high-density cultures constrains FSH stimulation of aromatase activity. The last two observations made suggest the existence of a dynamic interplay between microfilament organization and FSH action in Sertoli cells.
S B Meroni; H F Schteingart; E H Pellizzari; S B Cigorraga
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  112     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-12-15     Completed Date:  1995-12-15     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  69-75     Citation Subset:  IM    
Centro de Investigaciones Endocrinológicas, Hospital de Niños R. Gutiérrez, Buenos Aires, Argentina.
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MeSH Terms
Aromatase / metabolism*
Bucladesine / pharmacology
Cell Count
Cells, Cultured
Colchicine / pharmacology
Cytochalasin B / pharmacology
Follicle Stimulating Hormone / pharmacology
Microfilaments / drug effects,  physiology*
Microtubules / drug effects,  physiology
Rats, Sprague-Dawley
Sertoli Cells / enzymology*,  ultrastructure*
gamma-Glutamyltransferase / metabolism
Reg. No./Substance:
14930-96-2/Cytochalasin B; 362-74-3/Bucladesine; 64-86-8/Colchicine; 9002-68-0/Follicle Stimulating Hormone; EC; EC

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