| Possible involvement of intracellular angiotensin II receptor in high-glucose-induced damage in renal proximal tubular cells. | |
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MedLine Citation:
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PMID: 20890878 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: Recent studies have identified high glucose as a potent stimulus for the intracellular synthesis of angiotensin II. However, the exact roles of angiotensin II and angiotensin II type 1 receptor blockers (ARB) in high-glucose-induced renal tubular function remain unclear. Methods: N-Acetyl-beta-glucosaminidase (NAG), angiotensin II and 8-hydroxy-2'-deoxyguanosine (8-OHdG) concentrations in renal proximal tubular epithelial cells (RPTECs) with or without high glucose/ARB were determined using a modified commercial procedure. The changes of p22phox and cytoplasmic inhibitory kappa B (IkB) protein levels in RPTECs were measured using Western blotting. Results: High-glucose treatment (4x10-2 mol/L) significantly increased NAG release, angiotensin II concentrations in cell lysates and 8-OHdG and p22phox protein levels compared with those in regular glucose medium (1.75x10-2 mol/L). ARBs (candesartan, olmesartan or valsartan; 1x10-9-10-7 mol/L) showed a significant reduction in high-glucose-induced NAG, 8-OHdG and p22phox protein levels in RPTECs. Significant decreases of cytoplasmic IkB protein levels were observed in the high-glucose-treated group in RPTECs. ARBs markedly reversed high-glucose-induced reduction of IkB protein levels in RPTECs. Conclusions: ARBs reduce high-glucose-induced oxidative stress in RPTECs possibly via blockade of intracellular as well as extracellular AT1 receptor signaling, which possibly protects renal tubular cell function during diabetic nephropathy. |
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Authors:
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Toshihiro Takao; Taro Horino; Toru Kagawa; Reiko Matsumoto; Yoshiko Shimamura; Koji Ogata; Kousuke Inoue; Yoshinori Taniguchi; Takafumi Taguchi; Tatsuhito Morita; Yoshio Terada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of nephrology Volume: 24 ISSN: 1724-6059 ISO Abbreviation: J. Nephrol. Publication Date: 2011 Mar-Apr |
Date Detail:
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Created Date: 2011-03-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9012268 Medline TA: J Nephrol Country: Italy |
Other Details:
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Languages: eng Pagination: 218-24 Citation Subset: IM |
Affiliation:
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Division of Community Medicine, Department of Community Nursing, Kochi Medical School, ?Nankoku - Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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