Document Detail

Possible involvement of inhibitory GTP binding regulatory protein in alpha 2-adrenoceptor-mediated inhibition of adenylate cyclase activity in cerebral cortical membranes of rats.
MedLine Citation:
PMID:  2995588     Owner:  NLM     Status:  MEDLINE    
Influences of alpha 2-adrenoceptor stimulation on adenylate cyclase activity were investigated in cerebral cortical membranes of rats. Pretreatment of the membranes with islet-activating protein and NAD resulted in a significant increase in basal activity as well as in GTP- or forskolin/GTP-induced elevation of adenylate cyclase activity. Strong activation of adenylate cyclase was also caused in membranes pretreated with cholera toxin together with NAD in comparison to that in control membranes, suggesting that adenylate cyclase activity is perhaps regulated by stimulatory and inhibitory GTP binding regulatory protein existing in synaptic membranes. In addition, adrenaline (with propranolol) or clonidine significantly reduced adenylate cyclase activity stimulated by pretreatment with forskolin and GTP. The inhibitory effects of adrenaline were also observed in membranes pretreated with cholera toxin and NAD. Moreover, the inhibition by adrenaline or clonidine was completely abolished by treatment with (a) yohimbine or (b) islet-activating protein and NAD. It is suggested that alpha 2-receptor stimulation causes inhibitory influences on adenylate cyclase activity mediated by the inhibitory GTP binding regulatory protein in synaptic membranes of rat cerebral cortex.
Y Kitamura; Y Nomura; T Segawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  45     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1985-11-18     Completed Date:  1985-11-18     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1504-8     Citation Subset:  IM    
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MeSH Terms
Adenylate Cyclase / antagonists & inhibitors*
Adenylate Cyclase Toxin
Cell Membrane / drug effects,  metabolism
Cerebral Cortex / metabolism*
Cholera Toxin / pharmacology
Epinephrine / pharmacology
Forskolin / pharmacology
GTP-Binding Proteins / metabolism*
Guanosine Triphosphate / pharmacology
NAD / pharmacology
Pertussis Toxin
Rats, Inbred Strains
Receptors, Adrenergic, alpha / drug effects,  metabolism*
Virulence Factors, Bordetella / pharmacology
Reg. No./Substance:
0/Adenylate Cyclase Toxin; 0/Receptors, Adrenergic, alpha; 0/Virulence Factors, Bordetella; 51-43-4/Epinephrine; 53-84-9/NAD; 66428-89-5/Forskolin; 86-01-1/Guanosine Triphosphate; 9012-63-9/Cholera Toxin; EC Toxin; EC 3.6.1.-/GTP-Binding Proteins; EC Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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