Document Detail


Possible involvement of 4-hydroxynonenal in splenocyte regulated liver regeneration.
MedLine Citation:
PMID:  16403957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Liver regeneration is a complex, systemic process regulated by humoral and cellular mechanisms. Inflammatory response to the extensive tissue damage, as in partial hepatectomy, plays important role during regeneration. Hence, it is assumed that the spleen might play a role in systemic inflammatory response involved in liver regeneration. On the other hand, liver damage and consequential regeneration are often associated with oxidative stress and lipid peroxidation. One of the end products of lipid peroxidation, 4-hydroxynonenal (HNE), is nowadays considered not only as a "second toxic messenger of free radicals" but also as a growth-regulating factor. We therefore studied in vitro interactions of the HNE-treated murine liver cells and autologous spleen cells. The spleen cells supported recovery of liver cells from the HNE cytotoxicity although spleen cells themselves exerted cytotoxic effects against the proliferating liver cells that were not treated with HNE. Our results imply that the cytokines secreted by activated immunocompetent cells may be responsible for the observed recovery of the HNE-damage liver cells, suggesting that HNE might be an important factor regulating cellular and cytokine mediated mechanisms of liver regeneration control.
Authors:
Ana Cipak; Suzana Borovic; Mira Scukanec-Spoljar; Iva Kirac; Neven Zarkovic
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  BioFactors (Oxford, England)     Volume:  24     ISSN:  0951-6433     ISO Abbreviation:  Biofactors     Publication Date:  2005  
Date Detail:
Created Date:  2006-01-11     Completed Date:  2006-05-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807441     Medline TA:  Biofactors     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  217-26     Citation Subset:  IM    
Affiliation:
Rudjer Boskovic Institute, Zagreb, Croatia. acipak@irb.hr
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MeSH Terms
Descriptor/Qualifier:
Aldehydes / pharmacology*
Animals
Cell Count
Cell Survival
Coculture Techniques
DNA / biosynthesis
Female
Hepatocytes / metabolism,  ultrastructure
Lipid Peroxidation
Liver / drug effects,  metabolism,  ultrastructure
Liver Regeneration / drug effects*
Microscopy, Electron
Oxidative Stress
Rats
Rats, Wistar
Spleen / cytology,  physiology*
Thymidine / metabolism
Tritium
Chemical
Reg. No./Substance:
0/Aldehydes; 10028-17-8/Tritium; 29343-52-0/4-hydroxy-2-nonenal; 50-89-5/Thymidine; 9007-49-2/DNA

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