Document Detail


Possible inhibitory function of endogenous 15-hydroperoxyeicosatetraenoic acid on prostacyclin formation in bovine aortic endothelial cells.
MedLine Citation:
PMID:  3081039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Arachidonic acid is metabolized via the cyclooxygenase pathway to several potent compounds that regulate important physiological functions in the cardiovascular system. The proaggregatory and vasoconstrictive thromboxane A2 produced by platelets is opposed in vivo by the antiaggregatory and vasodilating activity of prostacyclin (prostaglandin I2) synthesized by blood vessels. Furthermore, arachidonic acid is metabolized by lipoxygenase enzymes to different isomeric hydroxyeicosatetraenoic acids (HETE's). This metabolic pathway of arachidonic acid was studied in detail in endothelial cells obtained from bovine aortae. It was found that this tissue produced 6-ketoprostaglandin F1 alpha as a major cyclooxygenase metabolite of arachidonic acid, whereas prostaglandins F2 alpha and E2 were synthesized only in small amounts. The monohydroxy fatty acids formed were identified as 15-HETE, 5-HETE, 11-HETE and 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT). The latter two compounds were produced by cyclooxygenase activity. Nordihydroguaiaretic acid (NDGA), a rather selective lipoxygenase inhibitor and antioxidant blocked the synthesis of 15- and 5-HETE. It also strongly stimulated the cyclooxygenase pathway, and particularly the formation of prostacyclin. This could indicate that NDGA might exert its effect on prostacyclin levels by preventing the synthesis of 15-hydroperoxyeicosatetraenoic acid (15-HPETE), a potent inhibitor of prostacyclin synthetase. 15-HPETE could therefore act as an endogenous inhibitor of prostacyclin production in the vessel wall.
Authors:
B Mayer; R Moser; H Gleispach; W R Kukovetz
Related Documents :
6413249 - Reticulocyte lipoxygenase exhibits both n-6 and n-9 activities.
11549379 - Inhibition of na(+)/h(+) exchange by sm-20220 attenuates free fatty acid efflux in rat ...
2981259 - Inhibition of cytotoxic t lymphocyte-mediated lysis by etya: effect independent of arac...
10914639 - Inhibition of cystic fibrosis transmembrane conductance regulator chloride channel curr...
3925129 - Autoregulatory vasodilation enhances renal prostaglandin e2 and associated renin releas...
21229319 - Disorders of bile acid synthesis.
19104179 - Cu-2010--a novel small molecule protease inhibitor with antifibrinolytic and anticoagul...
16292869 - Stereoselective total synthesis of cis- and trans-3-hydroxypipecolic acid.
20714659 - Efficient conversion of triacylglycerols and fatty acids to biodiesel in a microwave re...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  875     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1986 Feb 
Date Detail:
Created Date:  1986-04-02     Completed Date:  1986-04-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  641-53     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Aorta, Thoracic / metabolism
Arachidonic Acid
Arachidonic Acids / metabolism,  physiology*
Blood Vessels / metabolism*
Catechols / pharmacology
Cattle
Endothelium / metabolism
Epoprostenol / biosynthesis*
Fatty Acids / metabolism
Gas Chromatography-Mass Spectrometry
Leukotrienes*
Lipid Peroxides / physiology*
Nordihydroguaiaretic Acid
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Catechols; 0/Fatty Acids; 0/Leukotrienes; 0/Lipid Peroxides; 35121-78-9/Epoprostenol; 500-38-9/Nordihydroguaiaretic Acid; 506-32-1/Arachidonic Acid; 67675-14-3/15-hydroperoxy-5,8,11,13-eicosatetraenoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Biosynthesis of the sulfatide/GM1 activator protein (SAP-1) in control and mutant cultured skin fibr...
Next Document:  Distribution of apolipoproteins A-I and A-IV among lipoprotein classes in rat mesenteric lymph, frac...