| Possibility of islet transplantation from a nonheartbeating donor pancreas resuscitated by the two-layer method. | |
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MedLine Citation:
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PMID: 16210959 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The shortage of cadaveric donors is a problem in islet transplantation, and recent improvements in this field have led to renewed interest in the use of nonheartbeating (NHB) donors. NHB donor pancreata that could provide a significant source for islet transplantation are associated with warm ischemic injury. We tested whether the two-layer method (TL) could improve islet yield and function from damaged pancreata after warm ischemia (WI). METHODS: Lewis rats were divided into six groups. In groups 1 to 3, rats were subjected to 0, 30, and 45 minutes of WI, respectively. Islets were isolated immediately (subgroup a) or after 3-hour preservation with TL (subgroup b). Isolated islets were assessed in terms of islet yield and in vivo function. We also assessed the pancreatic tissue ATP concentration before isolation and distended pancreata morphologically after chemical digestion by H&E staining. RESULTS: Islet yield decreased significantly after 30 minutes of WI in group 2a, whereas TL preservation doubled this decreased yield in group 2b. Forty-five minutes of WI resulted in nearly no islet yield in both groups 3a and 3b. The success rates of transplantation in groups 1a, 1b, 2a, and 2b were 100%, 100%, 0%, and 75%, respectively. Increased tissue ATP levels and alleviation of morphological islet damage were observed in group 2b. CONCLUSIONS: These results demonstrated that pancreata damaged from 30-minute WI were restored by 3-hour TL preservation. TL may allow the selective use of NHB donors as an alternative source for islet transplantation. |
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Authors:
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Tomohiro Tanaka; Yasuyuki Suzuki; Yasuki Tanioka; Tetsuya Sakai; Keitaro Kakinoki; Tadahiro Goto; Shiri Li; Takuro Yoshikawa; Ippei Matsumoto; Yasuhiro Fujino; Yoshikazu Kuroda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Transplantation Volume: 80 ISSN: 0041-1337 ISO Abbreviation: Transplantation Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-10-07 Completed Date: 2005-12-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0132144 Medline TA: Transplantation Country: United States |
Other Details:
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Languages: eng Pagination: 738-42 Citation Subset: IM |
Affiliation:
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Division of Gastroenterological Surgery, Kobe University Graduate School of Medicine, Kobe, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Heart Arrest* Islets of Langerhans Transplantation / methods* Male Mice Pancreas / metabolism, pathology, physiology*, surgery* Rats Tissue Donors* Transplantation, Heterologous / methods |
| Chemical | |
Reg. No./Substance:
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56-65-5/Adenosine Triphosphate |
| Comments/Corrections | |
Comment In:
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Transplantation. 2006 May 27;81(10):1471-2; author reply 1472
[PMID:
16732188
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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