Document Detail


Possibility of skin epithelial cell transdifferentiation in tracheal reconstruction.
MedLine Citation:
PMID:  20946312     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
In tissue engineering, injured tissue is normally reconstructed with cells obtained from that tissue itself. However, it is difficult to obtain cells for reconstruction of the trachea because of its shape and limited accessibility. Therefore, other cell sources having similar form and function or stem cells are used for tracheal reconstruction. In a previous study, we used autologous skin epithelial cells and successfully reconstructed canine tracheas. We found that the tracheal epithelial layer was completely covered with ciliated cells, which is a remarkable finding because skin and tracheal epithelial cells originate from different germinal layers and have very different forms. In this study, to elucidate the origin of the ciliated cells, we identified the stem cell contents of skin epithelial cells on primary culture, marked the skin epithelial cells with PKH26 dye, and transplanted them onto canine tracheas. After 5 months, we identified PKH26 fluorescence on the tracheal epithelial layers, especially over the tracheal cartilages. Consequently, we demonstrated that transplanted autologous skin epithelial stem cells can remain viable on the trachea for a few months and can transdifferentiate into tracheal epithelial cells and chondrocytes.
Authors:
Jin Hoon Kim; Won Ho Kong; Jhin Gook Kim; Ho Joong Kim; Soo Won Seo
Publication Detail:
Type:  Journal Article     Date:  2010-10-14
Journal Detail:
Title:  Artificial organs     Volume:  35     ISSN:  1525-1594     ISO Abbreviation:  Artif Organs     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  United States    
Other Details:
Languages:  eng     Pagination:  122-30     Citation Subset:  IM    
Copyright Information:
© 2010, Copyright the Author. Artificial Organs © 2010, International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.
Affiliation:
SungKyunKwan University, School of Medicine, Biomedical Engineering Interdisciplinary Course, Samsung Biomedical Research Institute; and Departments of Thoracic and Cardiovascular Surgery and Pulmonary Medicine, Samsung Medical Center, Seoul, Korea.
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