Document Detail

Positive effect of silymarin on cell growth and differentiation in bovine and murine mammary cells.
MedLine Citation:
PMID:  19207679     Owner:  NLM     Status:  MEDLINE    
Silymarin, a naturally acknowledged hepatoprotector used in humans to treat liver diseases has been tested in murine (HC11) and bovine (BME-UV) mammary epithelial cell lines to evaluate a possible direct effect on cell growth and differentiation in mammary gland. Silymarin enhanced cell proliferation (p < 0.05) from 10 to 1000 ng/ml in association with growth factors, (up to 20%) or alone (up to 15%) versus controls. Furthermore, silymarin (100 ng/ml) was able to increase (p < 0.05) beta-casein gene expression alone or in association with prolactin (5 microg/ml). These effects may be related with protein kinase B (AKT) activation induced by silymarin treatment (p < 0.05) and/or by a dose-related inhibitory effect (p < 0.05) on caspase-3 activity related to a protective role in cell apoptosis. These data suggest that silymarin should be considered a candidate to support mammary gland activity during a lactogenetic state.
L Starvaggi Cucuzza; M Motta; S Miretti; E Macchi; E Martignani; P Accornero; M Baratta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-13
Journal Detail:
Title:  Journal of animal physiology and animal nutrition     Volume:  94     ISSN:  1439-0396     ISO Abbreviation:  J Anim Physiol Anim Nutr (Berl)     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-05-14     Completed Date:  2010-08-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101126979     Medline TA:  J Anim Physiol Anim Nutr (Berl)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  111-7     Citation Subset:  IM    
Department of Veterinary Morphophysiology, University of Torino, Grugliasco, Italy.
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MeSH Terms
Antioxidants / pharmacology
Caseins / genetics,  metabolism
Caspase 3 / metabolism
Cell Differentiation / drug effects*
Cell Enlargement
Cell Line
Epithelial Cells / cytology*,  drug effects*
Gene Expression Regulation / drug effects
Mammary Glands, Animal / cytology*
Prolactin / pharmacology
Signal Transduction
Silymarin / pharmacology*
Reg. No./Substance:
0/Antioxidants; 0/Caseins; 0/Silymarin; 9002-62-4/Prolactin; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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