| Positive effect of partial zona pellucida digestion on in vitro fertilization of mouse oocytes with cryopreserved spermatozoa. | |
| | |
MedLine Citation:
|
PMID: 18987060 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cryopreservation of mouse spermatozoa has been widely used; however, fertility of frozen spermatozoa in some strains, especially when inseminating cryopreserved oocytes, is low and may be improved by assisted fertilization techniques. The present study was performed to investigate the effect of partial zona pellucida (ZP) digestion on the in vitro fertilization (IVF) capacity of frozen mouse spermatozoa. Mouse oocytes were subjected to partial ZP digestion using acidic Tyrode's solution (pH 3.1). Fertilization rates in digestion groups (30 or 45 s) were higher (P < 0.05) than that of zona-intact control (78.3% or 86.3% vs. 52.5%). The recovery rate at 45 s was lower (P < 0.05) than that at 30 s (84.2% vs. 97.3%). Among vitrified oocytes, the fertilization rate in treatment group (digested for 30 s) was higher (P < 0.05) than that of zona-intact group (50.8% vs. 22.1%). After embryo transfer at the two-cell stage, 17.7% and 11.8% of transferred embryos derived from fresh and vitrified digested oocytes developed to term and showed no significant difference as compared with that from zona-intact oocytes (24.1%, P > 0.05). These results indicate that partial ZP digestion improves IVF efficiency of fresh and vitrified oocytes with frozen mouse spermatozoa, which can provide valuable information for in vitro assisted fertilization using cryopreserved gametes in the re-establishment of mouse colonies. |
| | |
Authors:
|
Zhi Qiang Fan; Yan Ping Wang; Chang Liang Yan; Lun Suo; Shi En Zhu |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-05 |
Journal Detail:
|
Title: Laboratory animals Volume: 43 ISSN: 0023-6772 ISO Abbreviation: Lab. Anim. Publication Date: 2009 Jan |
Date Detail:
|
Created Date: 2009-01-14 Completed Date: 2009-04-09 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0112725 Medline TA: Lab Anim Country: England |
Other Details:
|
Languages: eng Pagination: 72-7 Citation Subset: IM |
Affiliation:
|
College of Animal Science and Technology, China Agricultural University, Beijing, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cryopreservation* Female Fertilization / drug effects, physiology Fertilization in Vitro / methods* Hydrogen-Ion Concentration Isotonic Solutions / pharmacology Male Mice* Oocytes / drug effects Semen Preservation Spermatozoa* Time Factors Zona Pellucida / drug effects* |
| Chemical | |
Reg. No./Substance:
|
0/Isotonic Solutions; 0/Tyrode's solution |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Analysis of breeding and pathology helps refine management practices of a large-scale N'-ethyl-N'-ni...
Next Document: Coexistence of different tissue tumourigenesis in an N-methyl-N-nitrosourea-induced mammary carcinom...