| Positioning of bone marrow hematopoietic and stromal cells relative to blood flow in vivo: serially reconstituting hematopoietic stem cells reside in distinct nonperfused niches. | |
| | |
MedLine Citation:
|
PMID: 20393133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Hematopoietic stem cell (HSC) niches have been reported at the endosteum or adjacent to bone marrow (BM) vasculature. To investigate functional attributes of these niches, mice were perfused with Hoechst 33342 (Ho) in vivo before BM cell collection in presence of pump inhibitors and antibody stained. We report that the position of phenotypic HSCs, multipotent and myeloid progenitors relative to blood flow, follows a hierarchy reflecting differentiation stage, whereas mesenchymal stromal cells are perivascular. Furthermore, during granulocyte colony-stimulating factor-induced mobilization, HSCs migrated closer to blood flow, whereas stromal cells did not. Interestingly, phenotypic Lin(-)Sca1(+)KIT(+)CD41(-)CD48(-)CD150(+) HSCs segregated into 2 groups (Ho(neg) or Ho(med)), based on degree of blood/Ho perfusion of their niche. HSCs capable of serial transplantation and long-term bromodeoxyuridine label retention were enriched in Ho(neg) HSCs, whereas Ho(med) HSCs cycled more frequently and only reconstituted a single host. This suggests that the most potent HSC niches are enriched in locally secreted factors and low oxygen tension due to negligible blood flow. Importantly, blood perfusion of niches correlates better with HSC function than absolute distance from vasculature. This technique enables prospective isolation of serially reconstituting HSCs distinct from other less potent HSCs of the same phenotype, based on the in vivo niche in which they reside. |
| | |
Authors:
|
Ingrid G Winkler; Valérie Barbier; Robert Wadley; Andrew C W Zannettino; Sharon Williams; Jean-Pierre Lévesque |
Related Documents
:
|
15241173 - Microspheres accurately predict regional bone blood flow. 8029533 - Are laboratory values in bone marrow aspirate predictable for venous blood in paediatri... 2916623 - Blood glucose and oxygen tension levels in small-for-gestational-age fetuses. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-14 |
Journal Detail:
|
Title: Blood Volume: 116 ISSN: 1528-0020 ISO Abbreviation: Blood Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-07-23 Completed Date: 2010-08-20 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
|
Languages: eng Pagination: 375-85 Citation Subset: AIM; IM |
Affiliation:
|
Biotherapy Program, Mater Medical Research Institute, South Brisbane, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Benzimidazoles Blood Flow Velocity Bone Marrow / blood supply*, drug effects Bone Marrow Cells / cytology, physiology Bromodeoxyuridine / metabolism Cell Differentiation Cell Hypoxia Fluorescent Dyes Granulocyte Colony Stimulating Factor, Recombinant / administration & dosage Hematopoietic Stem Cell Mobilization Hematopoietic Stem Cells / cytology*, physiology Mice Mice, Congenic Mice, Inbred C57BL Multipotent Stem Cells / cytology, physiology Myeloid Progenitor Cells / cytology, physiology Phenotype Stromal Cells / cytology, physiology |
| Chemical | |
Reg. No./Substance:
|
0/Benzimidazoles; 0/Fluorescent Dyes; 0/Granulocyte Colony Stimulating Factor, Recombinant; 23491-52-3/HOE 33342; 59-14-3/Bromodeoxyuridine |
| Comments/Corrections | |
Comment In:
|
Blood. 2010 Jul 22;116(3):307-8
[PMID:
20651077
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Risk-adapted treatment of acute promyelocytic leukemia based on all-trans retinoic acid and anthracy...
Next Document: n-3 Polyunsaturated Fatty Acids Suppress Mitochondrial Translocation to the Immunologic Synapse and ...