Document Detail


Positional enhancer-blocking activity of the chicken beta-globin insulator in transiently transfected cells.
MedLine Citation:
PMID:  10588709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is thought that insulators demarcate transcriptionally and structurally independent chromatin domains. Insulators are detected by their ability to block enhancer-promoter interactions in a directional manner, and protect a transgene from position effects. Most studies are performed in stably transformed cells or organisms. Here we analyze the enhancer-blocking activity of the chicken beta-globin insulator in transient transfection experiments in both erythroid and nonerythroid cell lines. We show that four tandem copies of a 90-bp fragment of this insulator were able to block an enhancer in these experiments. In circular plasmids, placement on either side of the enhancer reduced activity, but when the plasmid was linearized, the enhancer-blocking activity was observed only when the insulator was placed between the promoter and the enhancer. These observations are consistent with the position-dependent enhancer-blocking activity of the insulator observed in stable transformation experiments.
Authors:
F Recillas-Targa; A C Bell; G Felsenfeld
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  96     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-01-05     Completed Date:  2000-01-05     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  14354-9     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. frecilla@ifisiol.uman.mx
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Cell Line
Chickens
Enhancer Elements, Genetic*
Erythrocytes / metabolism
Globins / genetics
Hydrogen-Ion Concentration
Plasmids
Promoter Regions, Genetic
Transfection*
Chemical
Reg. No./Substance:
9004-22-2/Globins
Comments/Corrections

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