| Portal vein embolization with radiolabeled polyvinyl alcohol particles in a swine model: hepatic distribution and implications for pancreatic islet cell transplantation. | |
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MedLine Citation:
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PMID: 19330373 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The distribution of radiolabeled polyvinyl alcohol microspheres (PVAMs) when infused into the portal vein of domestic swine was investigated, with the purpose of assessing implications for pancreatic islet cell transplantation. PVAMs measuring 100-300 microm (Contour SE) and labeled with (99m)Tc were infused into the main portal vein of 12 swine, with intermittent portal venous pressure measurements. The infusion catheter was introduced antegradely via direct or indirect cannulation of the portal vein. The liver was subsequently divided into anatomical segments. Radioactivity (decay corrected) was measured for (99m)Tc microsphere synthesis, dose preparation, gross organ activities, tissue samples, and blood. Particulate labeling, catheter positioning, and infusion were successful in all cases. The number of particles used was (185,000 +/- 24,000) with a volume of 1 ml. Mean portal pressure at 5 min was significantly higher than baseline, but without a significant difference at 15 min. Extrahepatic tissue and serum radioactivity was negligible. A significant difference in number of radioactive particles per gram was detected between segments 6/7 and segments 5/8. Intrasegmental activity was analyzed, and for segments 2/3 a significant difference in the percentage dose per gram across samples was demonstrated (P = 0.001). Effective and stable radiolabeling of PVAMs with (99m)Tc-sulfur colloid was demonstrated. Portal venous infusion of 100- to 300-microm particles showed entrapment in the sinusoidal hepatic system with transient portal pressure elevation. Preferential embolization into the right lateral and posterior segments occurs, suggesting that flow dynamics/catheter tip position plays a role in particle distribution. |
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Authors:
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Richard J Owen; John R Mercer; Faisal Al-Saif; Michele Molinari; Robert A Ashforth; Ray V Rajotte; Barbara Conner-Spady; A M James Shapiro |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-28 |
Journal Detail:
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Title: Cardiovascular and interventional radiology Volume: 32 ISSN: 1432-086X ISO Abbreviation: Cardiovasc Intervent Radiol Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-05-15 Completed Date: 2009-08-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8003538 Medline TA: Cardiovasc Intervent Radiol Country: United States |
Other Details:
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Languages: eng Pagination: 499-507 Citation Subset: IM |
Affiliation:
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Department of Radiology and Diagnostic Imaging, Faculty of Medicine, Walter Mackenzie Health Sciences Center, University of Alberta, 8440 112th Street, Edmonton, AB, T6G 2B7, Canada. drrichardowen@tbwifi.ca |
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Angiography Animals Contrast Media / administration & dosage, pharmacokinetics Embolization, Therapeutic / methods* Iohexol / administration & dosage, pharmacokinetics Islets of Langerhans Transplantation* Liver / metabolism* Microspheres Models, Animal Polyvinyl Alcohol / administration & dosage, pharmacokinetics* Portal Vein* Swine Technetium Tc 99m Sulfur Colloid / administration & dosage, pharmacokinetics |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 0/Technetium Tc 99m Sulfur Colloid; 66108-95-0/Iohexol; 9002-89-5/Polyvinyl Alcohol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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