Document Detail


Portal vein arterialization increases hepatocellular apoptosis and inhibits liver regeneration.
MedLine Citation:
PMID:  18599086     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Portal vein arterialization is performed in particular situations to guarantee sufficient blood flow in the portal vein. In addition, some authors have postulated a proliferation-promoting influence of portal vein arterialization on the liver tissue. However, portal vein arterialization is an unphysiological procedure: It increases portal blood flow and blood pressure as well as oxygenation of the liver tissue. On the other hand, it reduces the influx of hepatotrophic factors from the portal venous blood. The aim of these experiments was to investigate apoptosis and proliferation of hepatocytes during various conditions of the portal perfusion. MATERIALS AND METHODS: After 70% liver resection in Lewis rats, the following four experimental groups were formed differing in portal perfusion: (I) hyperperfused, nonarterialized; (II) flow-regulated, nonarterialized; (III) hyperperfused, arterialized; (IV) flow-regulated, arterialized. A warm ischemia of 30 min was kept in all groups. RESULTS: Portal vein arterialization of 70% reduced rat livers significantly reduced liver regeneration as shown by a significant reduction in liver weight, body weight, and liver function after 6 wk, in contrast to the group with 70% liver mass reduction and portal venous inflow of the portal vein. Furthermore, we found a significantly elevated number of apoptotic hepatocytes after portal vein arterialization. These results were independent from blood flow regulation of the arterialized portal vein, which caused no improvement of the results. CONCLUSIONS: Portal vein arterialization should be performed only temporarily and is clinically not recommended as a permanent option, because of the increased hepatocellular apoptosis and the very distinctive, negative long-term effects on liver weight.
Authors:
Karina Schleimer; Dirk L Stippel; Hans U Kasper; Klaus Prenzel; Cindy Gaudig; Samir Tawadros; Arnulf H Hoelscher; K Tobias E Beckurts
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-29
Journal Detail:
Title:  The Journal of surgical research     Volume:  149     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-15     Completed Date:  2008-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  250-8     Citation Subset:  IM    
Affiliation:
Department of Visceral and Vascular Surgery, University of Cologne, Cologne, Germany. Karina.Schleimer@uk-koeln.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Cell Proliferation
Hepatocytes / physiology*
Liver Regeneration*
Male
Portacaval Shunt, Surgical*
Portal Vein / physiology*,  surgery
Rats
Rats, Inbred Lew

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