Document Detail


Portal hypertensive response to bradykinin in inflamed or cirrhotic rat livers is mediated by B2-type receptors.
MedLine Citation:
PMID:  11206315     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: We have shown that the portal hypertensive response to bradykinin in normal rats is mediated by B2 receptors. METHODS: By using isolated and exsanguinated rat liver perfusion, we studied the portal hypertensive response to bradykinin or des-Arg9-bradykinin (B1 agonist) in inflamed or cirrhotic rat livers. Livers were perfused with bovine serum albumin Krebs-Henseleit buffer (pH 7.4; 37 degrees C) at a constant flow rate, in the absence or presence of des-Arg9[Leu8]-bradykinin or HOE 140 (B1 and B2 receptor antagonists, respectively). Bradykinin (140 nmol) or des-Arg9-bradykinin was injected as a bolus via the afferent route to the liver. RESULTS: Basal perfusion pressure in liver-cirrhotic rats was higher than in normal rats. In normal, inflamed, or liver-cirrhotic rats, the presence of the B1 antagonist did not change the portal hypertensive response to bradykinin, while the B2 antagonist abolished this response. A 140-nmol dose of des-Arg9-bradykinin did not change the perfusion pressure; 700 nmol of this B1 agonist produced an insignificant perfusion pressure increase. The perfusion pressure increase induced by bradykinin in cirrhotic livers was lower than in normal livers. CONCLUSIONS: The portal hypertensive response to bradykinin in inflamed or cirrhotic rat livers is mediated by B2 receptors, but not B1 receptors, and there is a contracting hyporeactivity to bradykinin in cirrhotic rat livers.
Authors:
M R Loureiro-Silva; H M Molina; D R Borges
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of gastroenterology and hepatology     Volume:  16     ISSN:  0815-9319     ISO Abbreviation:  J. Gastroenterol. Hepatol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-02-02     Completed Date:  2001-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8607909     Medline TA:  J Gastroenterol Hepatol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  41-5     Citation Subset:  IM    
Affiliation:
Laboratory of Experimental Hepatology (Núcleo de Metabolismo Hepático), Universidade Federal de São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Bradykinin / pharmacology*
Carbon Tetrachloride
Hypertension, Portal / drug therapy*
Inflammation
Kallidin / analogs & derivatives*,  pharmacology*
Liver Cirrhosis / chemically induced,  metabolism*,  pathology
Male
Rats
Rats, Wistar
Receptors, Bradykinin / metabolism*
Turpentine
Chemical
Reg. No./Substance:
0/Receptors, Bradykinin; 342-10-9/Kallidin; 56-23-5/Carbon Tetrachloride; 58-82-2/Bradykinin; 71800-36-7/kallidin, des-Arg(10)-; 8006-64-2/Turpentine

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