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Portable microfluidic chip for detection of Escherichia coli in produce and blood.
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PMID:  22679370     Owner:  NLM     Status:  MEDLINE    
Pathogenic agents can lead to severe clinical outcomes such as food poisoning, infection of open wounds, particularly in burn injuries and sepsis. Rapid detection of these pathogens can monitor these infections in a timely manner improving clinical outcomes. Conventional bacterial detection methods, such as agar plate culture or polymerase chain reaction, are time-consuming and dependent on complex and expensive instruments, which are not suitable for point-of-care (POC) settings. Therefore, there is an unmet need to develop a simple, rapid method for detection of pathogens such as Escherichia coli. Here, we present an immunobased microchip technology that can rapidly detect and quantify bacterial presence in various sources including physiologically relevant buffer solution (phosphate buffered saline [PBS]), blood, milk, and spinach. The microchip showed reliable capture of E. coli in PBS with an efficiency of 71.8% ± 5% at concentrations ranging from 50 to 4,000 CFUs/mL via lipopolysaccharide binding protein. The limits of detection of the microchip for PBS, blood, milk, and spinach samples were 50, 50, 50, and 500 CFUs/mL, respectively. The presented technology can be broadly applied to other pathogens at the POC, enabling various applications including surveillance of food supply and monitoring of bacteriology in patients with burn wounds.
ShuQi Wang; Fatih Inci; Tafadzwa L Chaunzwa; Ajay Ramanujam; Aishwarya Vasudevan; Sathya Subramanian; Alexander Chi Fai Ip; Banupriya Sridharan; Umut Atakan Gurkan; Utkan Demirci
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-05-29
Journal Detail:
Title:  International journal of nanomedicine     Volume:  7     ISSN:  1178-2013     ISO Abbreviation:  Int J Nanomedicine     Publication Date:  2012  
Date Detail:
Created Date:  2012-06-08     Completed Date:  2012-12-10     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101263847     Medline TA:  Int J Nanomedicine     Country:  New Zealand    
Other Details:
Languages:  eng     Pagination:  2591-600     Citation Subset:  IM    
Bio-Acoustic-MEMS in Medicine Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA.
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MeSH Terms
Antibodies, Immobilized / metabolism
Blood / microbiology
Colony Count, Microbial
Escherichia coli / isolation & purification*,  metabolism
Food Microbiology / instrumentation*,  methods*
Microfluidic Analytical Techniques / instrumentation*,  methods*
Milk / microbiology
Reproducibility of Results
Sensitivity and Specificity
Spinacia oleracea / microbiology
Statistics, Nonparametric
Surface Properties
Grant Support
Reg. No./Substance:
0/Antibodies, Immobilized

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Int J Nanomedicine
Journal ID (iso-abbrev): Int J Nanomedicine
ISSN: 1176-9114
ISSN: 1178-2013
Publisher: Dove Medical Press
Article Information
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© 2012 Wang et al, publisher and licensee Dove Medical Press Ltd.
collection publication date: Year: 2012
Print publication date: Year: 2012
Electronic publication date: Day: 29 Month: 5 Year: 2012
Volume: 7First Page: 2591 Last Page: 2600
ID: 3368510
PubMed Id: 22679370
DOI: 10.2147/IJN.S29629
Publisher Id: ijn-7-2591

Portable microfluidic chip for detection of Escherichia coli in produce and blood
ShuQi Wang1*
Fatih Inci1*
Tafadzwa L Chaunzwa1
Ajay Ramanujam1
Aishwarya Vasudevan1
Sathya Subramanian1
Alexander Chi Fai Ip1
Banupriya Sridharan1
Umut Atakan Gurkan1
Utkan Demirci12
1Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02139
2Harvard-MIT Health Sciences and Technology, Cambridge, MA, USA
Correspondence: Correspondence: Utkan Demirci, Health Sciences and Technology, Harvard-MIT Health Sciences and Technology, Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, 65 Landsdowne St, No 267, 02139 Cambridge, MA, USA, Tel +1 650 9069227, Email
*These authors contributed equally to this work


Sepsis is a significant, life-threatening problem for military personnel and civilians alike. Clinicians typically make an initial diagnosis of infection with an intermittent examination observing changes in temperature, blood pressure, smell, and sight. Depending on the severity of the inflicted wound and infectious agent, this may cause multiple organ dysfunction, hypoperfusion, failure of body systems (including the kidneys, liver, lungs, and central nervous system), and ultimately death. For sepsis diagnosis, daily culture and/or molecular analysis is performed using a small volume of the patient’s blood; usually less than 0.1% of entire blood volume (ie, approximately 5 mL). These detection methods are time-consuming and unreliable for identification of pathogens in up to 50% of septic patients. Following pathogen detection, the patient is treated with broad-spectrum antibiotics; this is not an ideal solution, because frequent use of broad-spectrum antibiotics also stimulates development of antibiotic-resistant bacteria. Antibiotic-resistant bacteria are a significant problem for injured military personnel and military treatment facilities. Currently, the sepsis mortality rate is 30%–50% among civilians and is higher in patients with military conditions including burns, trauma, and surgery.1 Although sepsis biomarkers, inflammatory modulators, and new antibiotics have been identified in past sepsis research, improved identification of various targets has had minimal impact on sepsis related morbidity and mortality. Therefore, there is a significant clinical need for new detection and identification technologies in this area. According to the Centers for Disease Control and Prevention, food-borne diseases cause approximately 76 million illnesses, 325,000 hospitalizations, and 5,000 deaths in US alone each year.2 The US Department of Agriculture showed that medical costs, productivity losses, and costs of premature deaths caused by food-borne diseases are approximately $6.9 billion per year.3 In 2011, pathogen-based produce contamination triggered a global concern with the outbreak of the foodborne toxin ‘Shiga’, which is produced by Escherichia coli (E. coli).4 On the other hand, sepsis is the tenth leading cause of death in the US,5 amounting to 24,179 cases in 49 US hospitals over a period of 7 years.6 As reported, E. coli can contaminate food source7 and cause sepsis in burn patients.8,9 Thus, effective E. coli detection would have a positive impact.

Currently, the gold standard detection method for bacteria is agar plate culture. However, this method is limited by the culturing time and volume of sample required to determine the presence of pathogens (Figure 1). Due to the challenge of obtaining enough sample volume, agar plate cultures give false negative results at rates ranging from 7.2% to 21.2%.10,11 In addition, the process is complicated by the fact that clinical samples need to go through multiple post-cultural steps for analysis, including Giemsa staining and differentiation on MacConkey plates.12 The whole process takes 48 to 72 hours.13 Although polymerase chain reaction (PCR) has high sensitivity and specificity,14 the need for a thermal cycler makes it unsuitable for point-of-care (POC) testing.15 Therein lies the niche for which microfluidic technologies are ideal; they have been employed to develop POC testing devices because of low manufacturing cost, reduced consumption of samples and reagents, and shortened assay time.1520 However, existing microfluidic devices for bacterial detection, either based on PCR21 or enzyme-linked immunosorbent assay (ELISA), require multiple sample processing steps prior to detection.22,23 All of these methods suffer from challenges including culture time, need of high sample volumes and reagents, the requirement for preprocessing of samples, low accuracy of the pathogen detection, and high cost. Further, for the detection of rare bacteria, PCR and ELISA based methods require large initial sample volumes, and preprocessing of samples, and sample amplification. Thus, there is an unmet need to develop POC devices that can address these issues, and capture, isolate and detect bacteria from biologically complex samples such as blood and produce.

To address this unmet need, we developed a POC microchip for capture, isolation, and detection of E. coli in various samples such as physiological buffer solution (phosphate buffered saline [PBS]), blood, milk, and spinach in a simple and rapid manner. This microchip technology could be broadly used as a POC device for multiple applications to rapidly screen for bacteria contamination in blood and food samples, thus improving healthcare and food safety.

Materials and methods
Device fabrication

The microfluidic device was fabricated as previously reported.18,24,25 The device was designed with dimensions of 22 mm × 60 mm with three parallel microchannels. To assemble this device, poly(methyl methacrylate) (PMMA) (1.5 mm thick; McMaster Carr, Atlanta, GA) and double-sided adhesive film (DSA) (50 μm thick; iTapestore, Scotch Plains, NJ) were cut using a laser cutter (Versa Laser™, Scottsdale, AZ). The PMMA base and a glass cover slip were then assembled via the DSA. In the assembled E. coli detection device, three microchannels (with dimensions of 50 mm × 4 mm × 50 μm in the DSA layer) were formed with an inlet and outlet (0.565 mm in diameter) at each end of the channels in the DSA layer. Before assembling the chip, glass cover was cleaned with ethanol using sonication. Then, it was washed with distilled water and dried under nitrogen gas. After cleaning steps, the glass cover was plasma treated for 60 seconds. Then, PMMA, DSA, and glass cover were assembled to form the complete microchip (Figure 2A).

Strains used in studies: genetically modified E. coli

To validate the surface chemistry, a genetically modified E. coli strain expressing emerald green fluorescent protein (EmGFP) was used. The E. coli strain BL21 Star™, and EmGFP-expressing plasmid were purchased from Invitrogen (Carlsbad, CA). According to the manufacturer’s instructions, the pRSET/EmGFP plasmid, which confers ampicillin resistance and green fluorescence, (Invitrogen, catalog number V353–20) was transferred into BL21 Star™ by incubating at 41°C for 30 seconds and transferring onto ice. The transformed BL21 Star™ was then incubated at 37°C by shaking at 250 rpm for an hour in Super Optimal broth with catabolite repression medium. Following this step, BL21 Star™ was streaked onto Luria–Bertani (LB) agar plates containing 100 mg/mL of ampicillin, and then incubated at 37°C for 16 hours. An isolated E. coli colony was picked and inoculated in 5 mL of LB medium on another LB agar plate, with 100 mg/mL of ampicillin added. The E. coli culture was then incubated at 37°C with shaking at 250 rpm for 16 hours. The E. coli culture was then aliquoted, and used as a standard stock for all experiments. The E. coli stock was diluted ten-fold in PBS and spread on LB-ampicillin plates. After overnight incubation at 37°C, single colonies of E. coli were counted and the number was used to calculate the original concentration of the E. coli stock.

Surface chemistry

Two different surface chemistry methods were evaluated to immobilize antibodies for the highest capture efficiency of E. coli on chip (Figure 2B). Protein G is an immunoglobulin-binding protein and has the ability to immobilize the fragment crystallization (Fc) region of antibodies. NeutrAvidin has strong affinity for biotin-conjugated biomolecules (eg, biotinylated antibodies). In the first method, a biotinylated antibody was immobilized via NeutrAvidin-based surface chemistry.18 Briefly, a glass slide was first plasma treated to make the surface more hydrophilic. 200 mM of 3-mercaptopropyl-trimethoxysilane (100 μL) dissolved in ethanol was then injected through the channels and incubated for 30 minutes at room temperature. 2 mM of N-(gamma-maleimidobutyryloxy) succinimide ester (GMBS; 100 μL) in ethanol, a cross linker of proteins to antibodies, was then incubated in microchannels for 35 minutes at ambient temperature. An ethanol wash of 300 μL was performed to remove the excess of untreated reagents after each incubation step. Before NeutrAvidin binding on the microchannels, 300 μL of PBS was used to wash out ethanol from the chip surface. 30 μL of NeutrAvidin solution (1 mg/mL in PBS) was incubated for 1 hour at room temperature before capture antibody immobilization. Three different experimental designs were performed for the NeutrAvidin studies. In the first design, 30 μL of biotinylated lipopolysaccharide (LPS) binding protein (LBP) Ab solution (5 μg/mL), (goat immunoglobulin G antibody, R&D Systems, Minneapolis, MN) was subsequently immobilized on the microchannel surface. Then, 30 μL of carrier-free recombinant human LBP (R&D Systems) at a concentration of 10 μg/mL was coated on the top of biotinylated anti-LBP antibody (anti-LBP-LBP). In the second design, the same process as performed in the first design was followed by the addition of 30 μL of bovine serum albumin (BSA) to block any remaining reactive spaces on the surface (anti-LBP-LBP-BSA). In the third design, first LBP was directly bound to GMBS to test the effect of protein orientation on E. coli capture, and then the BSA blocking step was performed after LBP binding (LBP-BSA). LBP was incubated in microchannels for 1 hour at room temperature.

In the second surface chemistry method, three nonbiotinylated antibodies (ie, anti-LPS antibody, cluster of differentiation 14 protein (CD14) of human monocyte and antiflagellin antibody) were immobilized onto microchannels via Protein G.26 In this method, processing steps were same as the first method except for the use of Protein G instead of NeutrAvidin. The Protein G concentration used in the immobilization of alternate antibodies onto the surface was 1 mg/mL. Then 30 μL of 5 μg/mL anti-LPS antibody (Abcam, Cambridge, MA) was immobilized on the surface via Protein G. 30 μL of CD14 (Abcam, Cambridge, MA) was used at 5 μg/mL to capture E. coli by binding to LPS on E. coli surface. Movement of LPS monomers to a binding site on CD14 was catalyzed by LBP.27,28 Finally, 30 μL of antiflagellin antibody (BioLegend, San Diego, CA) was used at a concentration of 5 μg/mL to capture E. coli via the surface protein flagellin (part of flagellum).29 These three capture agents were simultaneously incubated in microchannels for 1 hour at room temperature.

Sample preparation

EmGFP-expressing E. coli was spiked into 1 × PBS, blood, milk, and spinach samples with the final concentrations ranging from 50 to 4,000 colony forming units (CFUs) per milliliter for analysis on chip. Discarded de-identified whole blood (purchased from Research Blood Components, LLC, Cambridge, MA) was used in this study. Whole blood was spiked with E. coli and then inverted gently for 1 hour to enable homogenous distribution of E. coli. Spinach and milk samples were obtained from a local grocery store. Spinach leaves were washed and then spiked with E. coli before thorough mixing in a blender with 100 mL of sterile deionized H2O. The resultant mixture containing E. coli was filtered through a cell strainer (70 μM) to remove the residues of spinach leaves prior to microchip testing. Milk was spiked with E. coli and vortexed for 1 minute before testing.

Device operation and on-chip detection

To optimize the capture efficiency, 75 μL of EmGFP-expressing E. coli sample was pipetted into the functionalized microchannels, and then incubated at ambient temperature for 30 minutes. Following the incubation, microchannels were washed with 300 μL of PBS at a flow rate of 2 μL/minute using a syringe pump (Harvard Apparatus, Holliston, MA) for 150 minutes. For further optimization of processing time 2, 5, and 10 μL/minute wash was performed. After washing, captured E. coli was imaged using an inverted fluorescence microscope (Carl Zeiss, Thornwood, NY) through a GFP fluorescence filter (excitation wavelength 470 nm). For comparison, bright field and fluorescence images of E. coli taken under 10× and 100× magnification were merged (Figure 2C). The number of E. coli detected under 10× magnification using a GFP filter was counted manually.

Statistical analysis

To evaluate the effect of the capture agent on capture efficiency, we analyzed the experimental results (n = 2–6) using analysis of variance with Tukey’s post-hoc test for multiple comparisons with statistical significance threshold set at 0.05 (P < 0.05). To evaluate the effect of flow rate on capture efficiency, we analyzed the experimental results (n = 3–8) using nonparametric Kruskal–Wallis test followed by pair-wise comparisons with nonparametric upper-tailed Mann–Whitney U test, with statistical significance threshold set at 0.05 (P < 0.05). When nonparametric pair-wise tests were performed, Bonferroni correction was used for multiple comparisons. The agreement between chip counts and the E. coli stock concentrations in different media (PBS, blood, milk, spinach) was evaluated by calculating Pearson product-moment correlation coefficient (r) with statistical significance threshold set at 0.05 (P < 0.05). The Bland–Altman comparison analysis was used to evaluate the repeatability of the chip counts using residual analysis in comparison to E. coli stock concentrations. The coefficient of repeatability was calculated as 1.96 times the standard deviations of the differences between chip measurements and E. coli stock concentrations. In the Bland–Altman analysis, a mean difference of zero indicates that the chip measurement is unbiased with respect to the standard. A clinically acceptable range indicates the interval within which the difference would fall approximately 95% of the time. If the mean difference and the limit of agreement are within the clinically acceptable range, then the developed measurement method is deemed comparable to the standard. Statistical analyses were performed with Minitab (Release 14; Minitab Inc, State College, PA).

Results and discussion

We engineered the surface chemistry using immobilized antibodies, where the performance of the chip relies on nanoscale reactions on the microchannel surface. We evaluated antibodies specific to E. coli surface proteins using commonly reported antibody immobilization methods that provide a uniform distribution of antibodies on the capture surface in microfluidic channels, ie, Protein G and NeutrAvidin based methods. To develop a rapid microchip method for E. coli detection, four different capturing agents were immobilized in microchannels via these two surface chemistry methods. As shown in Figure 2B, anti-LPS, antiflagellin, and CD14 were immobilized on the microchannel surface via Protein G and anti-LBP antibody was immobilized on the microchannel surface via NeutrAvidin. To investigate the binding of anti-LBP antibody, and to observe the effect of antibody orientation on E. coli capture. Three different experimental designs were performed. In NeutrAvidin experiments, capture efficiencies of anti-LBP-LBP, anti-LBP-LBP-BSA, and LBP-BSA were observed to be 71.8% ± 5%, 60.7% ± 2%, and 44.5% ± 5%, respectively (Figure 3A). In comparison, E. coli capture efficiencies of antiflagellin, anti-LPS and CD14 obtained by using Protein G based surface chemistry, were 46.9% ± 3%, 41.5% ± 5%, and 41.0% ± 2%, respectively (Figure 3A). The capture efficiency via anti-LBP-LBP was observed to be significantly greater (P < 0.05) than the other capture agents that were used with Protein G based surface chemistry (Figure 3A). In our prior study, Protein G and NeutrAvidin exhibited similar efficiency to immobilize capture agents on microchannel surfaces.26 Thus, the difference in capture efficiency of E. coli was mainly due to the affinity of capture agents, ie, anti-LPS, antiflagellin, CD14, and LBP. The highest capture efficiency (71.8% ± 5%) was observed in microchannels immobilized with anti-LBP in the presence of LBP. This observation is in accordance with a previous report, in which LBP was shown to bind E. coli with high affinity; anti-LBP antibody helped the protein to take favorable orientation for E. coli capture.27 BSA blocking was used to prevent nonspecific binding in microchannels in addition to preventing the binding of anti-LBP antibodies and LBP onto the succinimide group of GMBS. The use of BSA as a blocking agent did not result in a statistically significant difference in capture efficiency with both anti-LBP-LBP and anti-LBP-LBP-BSA. The LBP-BSA experiment showed that the orientation of the LBP protein is critical to capture of E. coli, which was supported by statistical analysis of experimental results as shown in Figure 3A. As an overall result, NeutrAvidin- mediated anti-LBP antibody-LBP immobilization performed on the microchannel surface to attain high capture efficiency of E. coli.

By investigating the effects of washing flow rate on E. coli capture efficiency, anti-LBP antibody based surface chemistry was further optimized. At the flow rates of 2, 5, and 10 μL/min, capture efficiencies were 70.7% ± 4%, 60.5% ± 3%, and 53.9% ± 8%, respectively (Figure 3B). Statistical analysis on experimental results indicated that flow rate had a significant effect on capture efficiency (nonparametric Kruskal–Wallis test), where 2 μL/min resulted in significantly greater (P < 0.05) capture efficiency compared to 10 μL/min. The lower efficiency observed at higher flow rates may be related to the correspondingly higher shear stress within the microchannels. Additionally, we used food dyes to visualize and qualitatively analyze the wash steps in microchannels. We observed that the selected flow rate (2 μL/min) achieved effective removal of food dye solution from microchannels (Figure 4).

To determine the microchip’s limit of detection for E. coli capture, we used LB agar plate culture as the gold standard for E. coli detection. We correlated agar plate results for a series of known concentrations of E. coli with the microchip capture results. E. coli concentration and microchip-based E. coli detection showed a positive correlation for E. coli concentrations ranging from 50 to 4,000 CFUs/mL (Figure 5). The linearity of the correlation indicates that microchip-based E. coli detection can be used as an alternative to agar plate culture.

Figure 5A shows a sensitivity of 50 CFUs/mL that was obtained by the microchip count in PBS. Various sample types including blood, milk, and spinach, were used for rapid on-chip detection of E. coli (Figure 5C, E, and G). The detection limits of E. coli in blood, milk, and spinach samples were observed to be 50, 50, and 500 CFUs/mL as compared to E. coli dilution count, respectively. According to Bland–Altman analysis, the mean biases were −70 CFUs/mL, −165 CFUs/mL, −163 CFUs/mL, and −1869 CFUs/ mL for PBS (50 to 500 CFUs/mL), blood (50 to 500 CFUs/ mL), milk (50 to 500 CFUs/mL), and spinach (500 to 4,000 CFUs/mL) samples, respectively (Figure 5B, D, F, and H). Compared to E. coli spiked in PBS, the capture efficiency and limit of detection slightly decreased in other samples due to the immunological and enzymatic components in blood, milk, and spinach. For instance, there is a significant amount of albumin present in blood; this is commonly used as a blocking agent in immunoassays, and as such may have had a similar effect on our experiment. Milk also tends to have excessive albumin, casein, and other proteins, which may cause further blocking of the antigen-antibody binding. As the data show, these blocking and enzymatic agents can affect the capture efficiency. E. coli detection in spinach samples showed higher limit-of-detection than milk and blood experiments, potentially due to this experiment requiring preprocessing steps to remove the residuals of spinach leaves prior to microchip testing. Preprocessing steps (eg, filtering) caused a loss in E. coli cells before detection. On the other hand, the presented microchip assay achieved a lower detection limit for similar assays than was previously reported by methods such as magnetic separation method, fluorescence staining, and electrical detection (104 CFUs/mL and 8 × 104 CFUs/mL, respectively).30,31 Since nucleic acid-based-detection methods require more extensive sample preprocessing, antibody based E. coli detection on a chip presents a simpler alternative method to nucleic acid-based amplification assays on a chip.32,33 The current culture-based systems are subject to giving false negatives since only a small sample can be cultured compared to the whole sample volume, eg, 0.1% of the total blood volume is cultured in sepsis patients. This leads to 7.2 to 21.2% of such cultures providing false negatives.10,11 With the microchip system, larger sample sizes can be processed by continuous flow, giving results much faster than a culture system. The sample can also be reprocessed within either the same or new microchannels; this would be expected to increase the number of pathogens captured from a single sample. However, for detection purposes, this would be not necessary granted that accurate detection results are provided by the chip. Capturing larger numbers of pathogens can be beneficial for other applications such as culturing and downstream genomic analysis.

In this study, we used E. coli as a model pathogen because E. coli have been extensively characterized, and as such it provides simple comparison to existing systems. We transformed BL21 Star™ with EmGFP-expressing plasmid and developed a microfluidic device to rapidly capture and quantify E. coli from various biological samples. The GFP-expressing E. coli was used to facilitate the quantification steps. The bacteria can be visualized either under a microscope in a laboratory setting, or for rapid counts in POC settings, with wide field of view lensless imaging systems for rapid counts for POC settings.20,24,25,34 Also, for characterization of E. coli capture from various biological samples, we employed a GFP-expressing strain for ease of detection under a fluorescence microscope. The extension from a GFP-expressing strain to wild-type strains can be simply achieved using an ELISA-based detection method, as previously reported.35 The characterized microchip can be coupled with POC detection technologies such as surface plasmon resonance,3638 on-chip ELISA,39 and lensless imaging20,24 to achieve POC testing without reference to laboratory equipment. Additionally, the presented microfluidic approach can be adapted to detect other microorganisms that cause sepsis such as Gram-negative bacteria (E. coli, Klebsiella, Pseudomonas, Enterobacter, and Hemophilus species) and Gram-positive bacteria (Staphylococcus, Streptococcus, and Enterococcus species).40 Also, the current material cost, without the labor and other costs of the microchip, includes 10¢ of glass, 1¢ of PMMA, and 70¢ of antibodies. The antibody cost could be lowered with large-scale production and ordering.

In conclusion, we presented a microfluidic immunoassay to capture E. coli from blood, milk and spinach samples. This microchip enables rapid detection of bacteria in blood samples and food supply. The technology can be potentially adapted for on-site real-time food quality monitoring and for diagnosis of sepsis at the POC.



The authors report no conflicts of interest in this work.


This work was made possible by a research grant that was awarded and administered by the US Army Medical Research and Materiel Command and the Telemedicine and Advanced Technology Research Center, at Fort Detrick, MD. We thank Drs Paul Nisson and John Carney for their feedback and productive discussions on the project. We would also like to thank Mr Noah MacCallum for his feedback.

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Article Categories:
  • Original Research

Keywords: Escherichia coli, microchip, sepsis, food safety, point-of-care.

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