Document Detail

Portable encapsidation signal of the L-A double-stranded RNA virus of S. cerevisiae.
MedLine Citation:
PMID:  2117501     Owner:  NLM     Status:  MEDLINE    
The (+) single-stranded RNA (ssRNA) of the L-A virus is the species packaged to form new viral particles. Empty L-A viral particles specifically bind viral (+) ssRNA, and a sequence 400 bases from the 3' end is necessary for this activity. We show that its stem-loop structure, the A residue protruding from the stem, and the loop sequence are all important for the binding, and that this 34 base region is sufficient for the binding. M1, a satellite virus of L-A, has a similar structure on its (+) strand that is likewise sufficient for the binding. Heterologous RNA with the binding sequence from L-A or M1, when expressed in vivo, was packaged in L-A viral particles. Thus, the sites necessary to bind to empty particles are encapsidation signals for the L-A virus. Since the pol domain of the 180 kd minor coat protein appears to be responsible for the binding, this result suggests that the RNA polymerase molecule recognizes the viral genome for packaging.
T Fujimura; R Esteban; L M Esteban; R B Wickner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  62     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-09-27     Completed Date:  1990-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  819-28     Citation Subset:  IM    
Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
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MeSH Terms
Base Sequence
Binding Sites
Capsid / metabolism
Carrier Proteins / physiology
Cloning, Molecular
Genes, Viral*
Molecular Sequence Data
Nucleic Acid Conformation
RNA Viruses / genetics*
RNA, Viral / genetics*,  ultrastructure
RNA-Binding Proteins
Regulatory Sequences, Nucleic Acid
Saccharomyces cerevisiae*
Viral Structural Proteins / genetics*
Virus Replication
Reg. No./Substance:
0/Carrier Proteins; 0/RNA, Viral; 0/RNA-Binding Proteins; 0/Viral Structural Proteins

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