| Portable, battery-operated, low-cost, bright field and fluorescence microscope. | |
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MedLine Citation:
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PMID: 20694194 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study describes the design and evaluation of a portable bright-field and fluorescence microscope that can be manufactured for $240 USD. The microscope uses a battery-operated LED-based flashlight as the light source and achieves a resolution of 0.8 microm at 1000x magnification in fluorescence mode. We tested the diagnostic capability of this new instrument to identify infections caused by the human pathogen, Mycobacterium tuberculosis. Sixty-four direct, decontaminated, and serially diluted smears were prepared from sputa obtained from 19 patients suspected to have M. tuberculosis infection. Slides were stained with auramine orange and evaluated as being positive or negative for M. tuberculosis with both the new portable fluorescence microscope and a laboratory grade fluorescence microscope. Concordant results were obtained in 98.4% of cases. This highly portable, low cost, fluorescence microscope may be a useful diagnostic tool to expand the availability of M. tuberculosis testing at the point-of-care in low resource settings. |
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Authors:
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Andrew R Miller; Gregory L Davis; Z Maria Oden; Mohamad Reza Razavi; Abolfazl Fateh; Morteza Ghazanfari; Farid Abdolrahimi; Shahin Poorazar; Fatemeh Sakhaie; Randall J Olsen; Ahmad Reza Bahrmand; Mark C Pierce; Edward A Graviss; Rebecca Richards-Kortum |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-04 |
Journal Detail:
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Title: PloS one Volume: 5 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2010 |
Date Detail:
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Created Date: 2010-08-09 Completed Date: 2010-11-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e11890 Citation Subset: IM |
Affiliation:
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Beyond Traditional Borders, Rice University, Houston, Texas, USA. andymill@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Electric Power Supplies* Humans Lighting / methods* Microscopy, Fluorescence / economics*, instrumentation* Mycobacterium tuberculosis / isolation & purification Sputum / microbiology |
| Grant Support | |
ID/Acronym/Agency:
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52005885//Howard Hughes Medical Institute |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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