| Porphyromonas gingivalis peptidoglycans induce excessive activation of the innate immune system in silkworm larvae. | |
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MedLine Citation:
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PMID: 20702417 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Porphyromonas gingivalis, a pathogen that causes inflammation in human periodontal tissue, killed silkworm (Bombyx mori, Lepidoptera) larvae when injected into the blood (hemolymph). Silkworm lethality was not rescued by antibiotic treatment, and heat-killed bacteria were also lethal. Heat-killed bacteria of mutant P. gingivalis strains lacking virulence factors also killed silkworms. Silkworms died after injection of peptidoglycans purified from P. gingivalis (pPG), and pPG toxicity was blocked by treatment with mutanolysin, a peptidoglycan-degrading enzyme. pPG induced silkworm hemolymph melanization at the same dose as that required to kill the animal. pPG injection increased caspase activity in silkworm tissues. pPG-induced silkworm death was delayed by injecting melanization-inhibiting reagents (a serine protease inhibitor and 1-phenyl-2-thiourea), antioxidants (N-acetyl-l-cysteine, glutathione, and catalase), and a caspase inhibitor (Ac-DEVD-CHO). Thus, pPG induces excessive activation of the innate immune response, which leads to the generation of reactive oxygen species and apoptotic cell death in the host tissue. |
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Authors:
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Kenichi Ishii; Hiroshi Hamamoto; Katsutoshi Imamura; Tatsuo Adachi; Mikio Shoji; Koji Nakayama; Kazuhisa Sekimizu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-11 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 285 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-18 Completed Date: 2010-11-24 Revised Date: 2011-10-24 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 33338-47 Citation Subset: IM |
Affiliation:
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Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / pharmacology Apoptosis / drug effects, immunology Bacteroidaceae Infections / immunology, microbiology Bombyx / immunology*, microbiology Cysteine Proteinase Inhibitors / pharmacology Hemolymph / immunology*, microbiology Humans Immunity, Innate / drug effects*, physiology Larva / immunology, microbiology Peptidoglycan / immunology*, pharmacology Periodontitis / immunology, microbiology Porphyromonas gingivalis / immunology*, pathogenicity Reactive Oxygen Species / immunology Serine Proteinase Inhibitors / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Cysteine Proteinase Inhibitors; 0/Peptidoglycan; 0/Reactive Oxygen Species; 0/Serine Proteinase Inhibitors |
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