| Porphyromonas gingivalis infection in pregnant mice is associated with placental dissemination, an increase in the placental Th1/Th2 cytokine ratio, and fetal growth restriction. | |
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MedLine Citation:
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PMID: 12933860 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our previous animal studies showed that maternal Porphyromonas gingivalis infection in a subcutaneous chamber is associated with hepatic and uterine translocation, as well as systemic induction of maternal inflammatory responses, both of which were associated with fetal growth restriction (FGR). However, P. gingivalis-challenged dams had fetuses with either FGR (2 standard deviations below mean weight of nonchallenged dams) or normal weight. Therefore, the objective of this study was to determine whether maternal infection with P. gingivalis compromises normal fetal development via direct placental invasion and induction of fetus-specific placental immune responses characterized by a proinflammatory Th1-type cytokine profile. P. gingivalis-specific DNA was detected in placentas and fetuses of FGR and normal littermates from P. gingivalis-infected dams. Th1- and Th2-type cytokine mRNA as well as tumor necrosis factor alpha and transforming growth factor beta 2 mRNA were examined in placental tissue by using reverse transcription-PCR to determine Th1/Th2 ratios. For eight litters containing both normal-weight and FGR fetuses, P. gingivalis DNA was detected only in the placentas of FGR fetuses. All fetuses and all amniotic fluid samples from infected and control dams were negative for P. gingivalis DNA. mRNA levels of gamma interferon and interleukin-2 (IL-2) were significantly increased in placentas of FGR fetuses, while expression of IL-10 was significantly decreased in the same group. These data indicate that, in P. gingivalis-challenged dams, within each litter there is placenta-specific translocation of P. gingivalis that results in growth restriction of the targeted fetus, which is associated with a shift in the placental Th1/Th2 cytokine balance. |
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Authors:
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Dongming Lin; Mary Alice Smith; John Elter; Catherine Champagne; Christine Lynn Downey; James Beck; Steven Offenbacher |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Infection and immunity Volume: 71 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 2003 Sep |
Date Detail:
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Created Date: 2003-08-22 Completed Date: 2003-09-29 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 5163-8 Citation Subset: IM |
Affiliation:
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Center for Oral and Systemic Diseases, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteroidaceae Infections / complications*, immunology, microbiology, pathology Cytokines / genetics Female Fetal Growth Retardation / etiology* Gene Expression Maternal-Fetal Exchange Mice Placenta / immunology, microbiology Porphyromonas gingivalis* / genetics, immunology, isolation & purification Pregnancy Pregnancy Complications, Infectious / immunology*, microbiology*, pathology RNA, Messenger / genetics, metabolism Th1 Cells / immunology Th2 Cells / immunology |
| Grant Support | |
ID/Acronym/Agency:
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R0-1 DE 12453/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/RNA, Messenger |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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