| Porphyromonas gingivalis infection accelerates intimal thickening in iliac arteries in a balloon-injured rabbit model. | |
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MedLine Citation:
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PMID: 18597601 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Current epidemiologic data suggest that a localized infection (periodontitis) can disseminate into the distant tissues, and subgingival bacteria can migrate in the bloodstream, thereby contributing to independent systemic disease processes. To test this hypothesis, we investigated the effect of repeated systemic inoculations with Porphyromonas gingivalis (Pg) on intimal hyperplasia in iliac arteries in a rabbit model of balloon injury. METHODS: One week after single balloon injury to the iliac artery, 30 male New Zealand rabbits were randomly assigned to intravenous inoculation with 100 microl live Pg (10(7) colony-forming units; n = 15) or vehicle (n = 15) once weekly for 4, 8, or 12 consecutive weeks. Arteries were fixed by perfusion and removed for analysis of neointimal lesion formation. We measured intimal and medial lesion areas in iliac artery cross-sections as well as the intimal/medial ratio (I/M). We also analyzed Pg 16S ribosomal DNA amplification with polymerase chain reaction, systemic proinflammatory mediators with enzyme-linked immunosorbent assay, and immunolocalization of macrophages in the balloon-injured arteries. RESULTS: At 12 weeks, iliac intimal hyperplasia was accelerated, and I/M was significantly increased in Pg-inoculated animals (I/M 3.961 +/- 0.536 in the Pg group versus 3.585 +/- 0.353 in the control animals; P <0.01). Pg-inoculated animals also had significant increases in macrophage infiltration at 12 weeks, C-reactive protein levels at all time points, and interleukin-6 levels at 12 weeks. Moreover, Pg ribosomal DNA was found in the injured arteries of Pg-inoculated animals, but only after 12 weeks. CONCLUSION: Long-term systemic challenge with Pg, an oral pathogen, may accelerate intimal hyperplasia in balloon-injured iliac arteries. |
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Authors:
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Ming-Zhu Zhang; Chao-Lun Li; Yun-Tao Jiang; Wei Jiang; Ying Sun; Rong Shu; Jing-Ping Liang |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of periodontology Volume: 79 ISSN: 0022-3492 ISO Abbreviation: J. Periodontol. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-03 Completed Date: 2008-10-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000345 Medline TA: J Periodontol Country: United States |
Other Details:
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Languages: eng Pagination: 1192-9 Citation Subset: D; IM |
Affiliation:
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Department of Endodontics and Operative Dentistry, The Ninth People's Hospital, School of Stomatology, Shanghai Jiaotong University, Shanghai, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacteroidaceae Infections / microbiology, physiopathology* Balloon Dilatation / adverse effects* C-Reactive Protein / analysis DNA, Bacterial / analysis DNA, Ribosomal / analysis Disease Models, Animal Hyperplasia Iliac Artery / injuries*, microbiology, pathology Inflammation Mediators / analysis Interleukin-6 / analysis Macrophages / pathology Male Porphyromonas gingivalis / physiology* RNA, Ribosomal, 16S / analysis Rabbits Random Allocation Time Factors Tunica Intima / injuries*, microbiology, pathology Tunica Media / pathology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Ribosomal; 0/Inflammation Mediators; 0/Interleukin-6; 0/RNA, Ribosomal, 16S; 9007-41-4/C-Reactive Protein |
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