Document Detail

Porosity of porcine bladder acellular matrix: impact of ACM thickness.
MedLine Citation:
PMID:  14613246     Owner:  NLM     Status:  MEDLINE    
The objectives of this study are to examine the porosity of bladder acellular matrix (ACM) using deionized (DI) water as the model fluid and dextran as the indicator macromolecule, and to correlate the porosity to the ACM thickness. Porcine urinary bladders from pigs weighing 20-50 kg were sequentially extracted in detergent containing solutions, and to modify the ACM thickness, stretched bladders were acellularized in the same manner. Luminal and abluminal ACM specimens were subjected to fixed static DI water pressure (10 cm); and water passing through the specimens was collected at specific time interval. While for the macromolecule porosity testing, the diffusion rate and direction of 10,000 MW fluoroescein-labeled dextrans across the ACM specimens mounted in Ussing's chambers were measured. Both experiments were repeated on the thin stretched ACM. In both ACM types, the fluid porosity in both directions did not decrease with increased test duration (3 h); in addition, the abluminal surface was more porous to fluid than the luminal surface. On the other hand, when comparing thin to thick ACM, the porosity in either direction was higher in the thick ACM. Macromolecule porosity, as measured by absorbance, was higher for the abluminal thick ACM than the luminal side, but this characteristic was reversed in the thin ACM. Comparing thin to thick ACM, the luminal side in the thin ACM was more porous to dextran than in the thick ACM, but this characteristic was reversed for the abluminal side. The porcine bladder ACM possesses directional porosity and acellularizing stretched urinary bladders may increase structural density and alter fluid and macromolecule porosity.
Walid Farhat; Jun Chen; Petar Erdeljan; Oren Shemtov; David Courtman; Antoine Khoury; Herman Yeger
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  67     ISSN:  1549-3296     ISO Abbreviation:  -     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-11-12     Completed Date:  2004-09-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  970-4     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 970-974, 2003
Department of Surgery, Division of Urology, The Hospital for Sick Children, M299, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8.
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MeSH Terms
Bioprosthesis / standards*
Hypotonic Solutions
Urinary Bladder* / anatomy & histology
Reg. No./Substance:
0/Hypotonic Solutions; 9004-54-0/Dextrans

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