| Porcine vascular smooth muscle cells immortalized with SV40 ori-defective DNA: characteristics of cell growth and collagen synthesis. | |
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MedLine Citation:
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PMID: 8237368 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A cell line derived from medial smooth muscle cells (SMC) was established from the porcine coronary artery by transfection with ori-defective simian virus 40 plasmid DNA (SV40 DNA). The characteristics of transfected cells (SV40-SMC) such as cell growth, collagen and non-collagen syntheses were investigated. SV40-SMC expressed SV40 large T antigen, c-myc and c-myb encoded proteins in the nuclei. SV40-SMC demonstrated a 'hills and valleys'-like arrangement in overconfluence and actin filaments upon immunofluorescent staining. Under electron microscopic observation, SV40-SMC had larger amounts of synthetic organelles and smaller amounts of filament bundles than those of SMC. SV40-SMC demonstrated three times higher growth activity and 4.4 times greater cellular density than SMC. Smooth muscle cells did not grow in media containing 5% plasma derived serum (PDS) instead of normal serum, whereas SV40-SMC proliferated in this medium. SV40-SMC did not grow in soft agar gel, while HeLa S3 cells, a cell line of human cervical carcinoma, formed colonies in this gel. By immunofluorescent (IF) staining, collagen phenotypes I, III, IV and V were detected in both SV40-SMC and SMC. However, protein synthesis including collagen and non-collagen was higher in SV40-SMC than in the control sample. It was concluded that SV40-SMC were a continuous cell line for vascular SMC regarding morphological characteristics, and demonstrated a higher growth activity, with increased collagen and non-collagen syntheses. This cell line is useful for the investigation of atherogenesis in relation to a proliferation of SMC and an accumulation of extracellular matrices in vascular intima. |
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Authors:
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K Fujimitsu; N Sakata; S Jimi; S Takebayashi; Y Sasaguri; M Morimatsu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta pathologica japonica Volume: 43 ISSN: 0001-6632 ISO Abbreviation: Acta Pathol. Jpn. Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1993-12-13 Completed Date: 1993-12-13 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372637 Medline TA: Acta Pathol Jpn Country: AUSTRALIA |
Other Details:
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Languages: eng Pagination: 481-9 Citation Subset: IM |
Affiliation:
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Second Department of Pathology, School of Medicine, Fukuoka University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agar Animals Antigens, Viral, Tumor / metabolism Cell Division Cell Line, Transformed Cells, Cultured Collagen / biosynthesis* Colony-Forming Units Assay DNA, Viral / genetics* Extracellular Matrix Proteins / biosynthesis Fluorescent Antibody Technique Gels Muscle, Smooth, Vascular / cytology*, metabolism* Oncogenes Simian virus 40 / genetics*, immunology Swine |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Viral, Tumor; 0/DNA, Viral; 0/Extracellular Matrix Proteins; 0/Gels; 9002-18-0/Agar; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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