Document Detail


Porcine skin-derived stem cells can serve as donor cells for nuclear transfer.
MedLine Citation:
PMID:  19226213     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although transgenic animal production through somatic cell nuclear transfer (SCNT) has been successful, the process is still inefficient. One major limitation is the use of somatic donor cells that have a finite life span. Identification and isolation of a cell type capable of rapid proliferation while possessing immortal or prolonged life span in culture and is capable of being genetically modified would be very valuable for utilization in the production of genetically modified pigs. Here we report the birth of live piglets after cloning by using porcine skin-derived stem cells (SSC) as a donor cell type. In the present study, cell cycle analysis indicates that the porcine SSC proliferate rapidly in vitro. The porcine SSC are capable of producing live offspring and can be genetically modified with positive selection. Utilization of porcine SSC may prove to be an excellent cell type for genetic modification followed by nuclear transfer for the production of transgenic pigs.
Authors:
Yanhong Hao; David Wax; Zhisheng Zhong; Clifton Murphy; Jason W Ross; August Rieke; Melissa Samuel; Lee Spate; Paul Dyce; Julang Li; Peter Sutovsky; Randall S Prather
Related Documents :
15102063 - Fm-dyes as experimental probes for dissecting vesicle trafficking in living plant cells.
1506433 - Syringe loading introduces macromolecules into living mammalian cell cytosol.
2468493 - Low level expression of cytokeratins 8, 18 and 19 in vascular smooth muscle cells of hu...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cloning and stem cells     Volume:  11     ISSN:  1557-7457     ISO Abbreviation:  Cloning Stem Cells     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-13     Completed Date:  2009-05-26     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  101125444     Medline TA:  Cloning Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  101-10     Citation Subset:  IM    
Affiliation:
University of Missouri, Columbia, 65211, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified*
Cell Culture Techniques
Cell Proliferation
Cloning, Organism / methods*
Microsatellite Repeats
Microscopy, Electron, Transmission
Nuclear Transfer Techniques*
Skin / cytology*
Stem Cells / cytology,  physiology*,  ultrastructure
Swine / genetics*
Grant Support
ID/Acronym/Agency:
R01-RR13438-10/RR/NCRR NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Human amniotic mesenchymal cells differentiate into chondrocytes.
Next Document:  Cloning missy: obtaining multiple offspring of a specific canine genotype by somatic cell nuclear tr...