Document Detail


Porcine nuclear transfer using somatic donor cells altered to express male germ cell function.
MedLine Citation:
PMID:  19698292     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies reported that the direct transformation of one differentiated somatic cell type into another is possible. In the present study, we were able to modulate the cell fate of somatic cells to take on male germ cell function by introducing cell extracts derived from porcine testis tissue. Fibroblasts were treated with streptolysin O, which reversibly permeabilises the plasma membrane, and incubated with testis extracts. Our results showed that the testis extracts (TE) could activate expression of male germ cell-specific genes, implying that TE can provide regulatory components required for altering the cell fate of fibroblasts. Male germ cell function was sustained for more than 10 days after the introduction of TE. In addition, a single TE-treated cell was injected directly into the cytoplasm of in vitro-matured porcine oocytes. The rate of blastocyst formation was significantly higher in the TE-treated nuclear donor cell group than in the control cell group. The expression level of Nanog, Sox9 and Eomes was drastically increased when altered cells were used as donor nuclei. Our results suggest that TE can be used to alter the cell fate of fibroblasts to express male germ cell function and improve the developmental efficiency of the nuclear transfer porcine embryos.
Authors:
Sangho Roh; Hye-Yeon Choi; Sang Kyu Park; Cheolhee Won; Bong-Woo Kim; Jung-Hyun Kim; Hoin Kang; Eung-Ryoung Lee; Ssang-Goo Cho
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction, fertility, and development     Volume:  21     ISSN:  1031-3613     ISO Abbreviation:  Reprod. Fertil. Dev.     Publication Date:  2009  
Date Detail:
Created Date:  2009-08-24     Completed Date:  2009-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907465     Medline TA:  Reprod Fertil Dev     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  882-91     Citation Subset:  IM    
Affiliation:
Embryo Biotechnology Laboratory, Dental Research Institute and CLS21, Seoul National University School of Dentistry, Seoul, Korea. sangho@snu.ac.kr; ssangoo@konkuk.ac.kr
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / pharmacology
Blastocyst / metabolism
Cell Membrane Permeability
Cell Transdifferentiation* / genetics
Cells, Cultured
Embryo Culture Techniques
Female
Fertilization in Vitro
Fibroblasts / drug effects,  metabolism*
Gene Expression Regulation, Developmental
Homeodomain Proteins / genetics
Male
Mice
Nuclear Transfer Techniques*
SOX9 Transcription Factor / genetics
Spermatozoa / drug effects,  metabolism*
Streptolysins / pharmacology
Swine
T-Box Domain Proteins / genetics
Testis / metabolism*
Time Factors
Tissue Extracts
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Homeodomain Proteins; 0/SOX9 Transcription Factor; 0/Streptolysins; 0/T-Box Domain Proteins; 0/Tissue Extracts; 0/streptolysin O

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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