Document Detail


Porcine leukocyte 5- and 12-lipoxygenases are iron enzymes.
MedLine Citation:
PMID:  1652456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
5- and 12-lipoxygenases isolated from porcine leukocytes were investigated by electron paramagnetic resonance at X-band and atomic absorption spectroscopy. For comparison potato 5-lipoxygenase was studied under identical experimental conditions. All three lipoxygenases contained between 0.7 and 0.9 Fe atoms/enzyme molecule. As isolated, both mammalian enzymes exhibited a characteristic EPR signal at low magnetic field with a maximum at g = 5.20 indicative of a high-spin ferric iron center. The signal was not affected by the oxidants 12-hydroperoxyeicosatetraenoic acid or arachidonic acid, nor was it affected by the reductant nordihydroguaiaretic acid. In the case of the potato enzyme an intense EPR signal with resonances at g = 7.50, 6.39 and 5.84 was only observed after addition of an oxidant, such as 9-hydroperoxyoctadecadienoic acid.
Authors:
P M Kroneck; C Cucurou; V Ullrich; N Ueda; H Suzuki; T Yoshimoto; S Matsuda; S Yamamoto
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  287     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1991 Aug 
Date Detail:
Created Date:  1991-09-27     Completed Date:  1991-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  105-7     Citation Subset:  IM    
Affiliation:
Fakultät für Biologie, Universität Konstanz, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonate 12-Lipoxygenase / analysis*,  chemistry
Arachidonate 5-Lipoxygenase / analysis*,  chemistry
Electron Spin Resonance Spectroscopy
Iron / analysis*
Leukocytes / enzymology*
Leukotrienes / pharmacology
Linoleic Acids / pharmacology
Lipoxygenase Inhibitors
Nordihydroguaiaretic Acid / pharmacology
Solanum tuberosum / enzymology
Soybeans / enzymology
Swine
Chemical
Reg. No./Substance:
0/Leukotrienes; 0/Linoleic Acids; 0/Lipoxygenase Inhibitors; 500-38-9/Nordihydroguaiaretic Acid; 63121-49-3/9-hydroperoxy-11,12-octadecadienoic acid; 67675-13-2/12-HPETE; 7439-89-6/Iron; EC 1.13.11.31/Arachidonate 12-Lipoxygenase; EC 1.13.11.34/Arachidonate 5-Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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