| Porcine B-cell activating factor promotes anti-FMDV antibodies in vitro but not in vivo after DNA vaccination of pigs. | |
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MedLine Citation:
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PMID: 17658618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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'B-cell activating factor belonging to the TNF family' (BAFF) represents a cytokine produced by antigen presenting cells promoting B-cell maturation, activation and immunoglobulin class switching. In the present study, we demonstrate expression of BAFF on cultured monocyte-derived dendritic cells, which is further enhanced by interferon-alpha or interferon-gamma treatment. From these cells, porcine BAFF was cloned and the recombinant protein was expressed in mammalian cells with and without a FLAG tag at the carboxyl terminus. Only the protein without the FLAG tag was bioactive in vitro, and promoted B-cell survival and the differentiation of foot-and-mouth disease virus (FMDV)-specific memory B cells into antibody producing cells. Based on this result it was tested whether BAFF can enhance FMDV antibody responses in the context of a DNA vaccination. To this end, pigs were immunised with the anti-FMDV DNA vaccine plasmid pcDNA3.1/P1-2A3C3D and a pCI plasmid expressing porcine BAFF. Using a needle-free transdermal application method, also referred to as 'jet injection', pigs were vaccinated three times and their humoral response quantified by ELISA and a virus neutralisation test. After the third vaccination, three out of six animals vaccinated with the pcDNA3.1/P1-2A3C3D alone but none of the animals that also received the BAFF expressing plasmid had seroconverted. These data suggest that BAFF is not appropriate as a genetic adjuvant when applied as a simple co-injection with the antigen-encoding plasmid. |
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Authors:
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Fabio Bergamin; Leslie Saurer; Viviane Neuhaus; Kenneth C McCullough; Artur Summerfield |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-22 |
Journal Detail:
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Title: Veterinary immunology and immunopathology Volume: 120 ISSN: 0165-2427 ISO Abbreviation: Vet. Immunol. Immunopathol. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-10-30 Completed Date: 2008-03-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8002006 Medline TA: Vet Immunol Immunopathol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 115-23 Citation Subset: IM |
Affiliation:
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Institute of Virology and Immunoprophylaxis, Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibodies, Viral / immunology* B-Cell Activating Factor / metabolism* Foot-and-Mouth Disease Virus / immunology* Gene Expression Regulation Models, Animal Molecular Sequence Data Recombinant Proteins Specific Pathogen-Free Organisms Swine / immunology* Vaccines, DNA / immunology* Viral Vaccines / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/B-Cell Activating Factor; 0/Recombinant Proteins; 0/Vaccines, DNA; 0/Viral Vaccines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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