Document Detail


Pool sizes of metabolic intermediates and their relation to glucose repression of beta-galactosidase synthesis in Escherichia coli.
MedLine Citation:
PMID:  4382255     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The intermediary metabolism of two strains of Escherichia coli has been examined. One strain (Q22) exhibits acute transient repression of beta-galactosidase synthesis when glucose is supplied to cells growing on glycerol; the other strain (W3110) does not. The two strains do not differ genetically in their lac operons. 2. Strain Q22 uses about twice as much glucose as strain W3110 per unit of cell mass produced. 3. Pentose phosphate-cycle activity in the presence of glucose is much stronger in strain Q22 than in strain W3110. 4. In strain Q22 the pool sizes of glucose 6-phosphate, 6-phosphogluconate, fructose 1,6-diphosphate and NADPH increase when glucose is added to cells growing on glycerol, and beta-galactosidase synthesis is severely inhibited. After about 1hr. the synthesis of beta-galactosidase is partly resumed, and the pool sizes of the four compounds fall. ATP, NADH and several other phosphorylated compounds show no concentration changes. 5. These concentration changes do not occur in strain W3110, in which beta-galactosidase synthesis is only rather weakly repressed by glucose. 6. It is suggested that repression of enzyme synthesis by glucose requires the rapid operation of the pentose phosphate cycle, and is mediated by one of the four substances whose concentration rises and later falls in strain Q22. A definite choice of effector from among these four possibilities cannot at present be made.
Authors:
C Prevost; V Moses
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  103     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1967 May 
Date Detail:
Created Date:  1967-10-04     Completed Date:  1967-10-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  349-57     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Bacteriological Techniques / instrumentation
Carbon Isotopes
Chromatography, Paper
Enzyme Induction
Enzyme Repression
Escherichia coli / enzymology*,  metabolism
Galactosidases / biosynthesis*
Genes
Glucose / metabolism*
Glycerol / metabolism
Hexosephosphates / metabolism
Kinetics
NAD / metabolism
NADP / metabolism
Species Specificity
Spectrophotometry
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Hexosephosphates; 50-99-7/Glucose; 53-59-8/NADP; 53-84-9/NAD; 56-65-5/Adenosine Triphosphate; 56-81-5/Glycerol; EC 3.2.1.-/Galactosidases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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