Document Detail


Polyvinyl alcohol and amino acids as substitutes for bovine serum albumin in culture media for mouse preimplantation embryos.
MedLine Citation:
PMID:  9286737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of replacing bovine serum albumin (BSA) in a simple defined medium (KSOM) with polyvinyl alcohol (PVA) and/or amino acids on the percentages of mouse zygotes that develop to at least the blastocyst stage and that hatch at least partially or completely is reported. Blastocysts could form when BSA was replaced with only PVA, but at a moderately reduced rate; however, partial hatching, and hence complete hatching, were severely impaired when BSA was replaced with only PVA. The substitution of BSA with amino acids alone resulted in a high rate of blastocyst formation and moderate impairment of hatching. The addition of PVA to BSA-free KSOM supplemented with amino acids had no extra effect. BSA had significant effects when added to BSA-free KSOM supplemented with amino acids. The BSA caused a significant increase in the rate of partial hatching, and may even have had a small effect on the rate of blastocyst formation. The results also showed that glucose, at a high concentration of 5.56 mM, does not inhibit the development of mouse zygotes to hatched blastocysts when cultured in KSOM supplemented with amino acids.
Authors:
J D Biggers; M C Summers; L K McGinnis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Human reproduction update     Volume:  3     ISSN:  1355-4786     ISO Abbreviation:  Hum. Reprod. Update     Publication Date:    1997 Mar-Apr
Date Detail:
Created Date:  1997-10-09     Completed Date:  1997-10-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9507614     Medline TA:  Hum Reprod Update     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  125-35     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / pharmacology*
Animals
Blastocyst / physiology*
Culture Media*
Culture Techniques
Embryonic Development*
Female
Glucose / pharmacology
Mice
Polyvinyl Alcohol / pharmacology*
Pregnancy
Serum Albumin, Bovine / pharmacology*
Zygote / growth & development
Grant Support
ID/Acronym/Agency:
UO1-HD21988/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 0/Serum Albumin, Bovine; 50-99-7/Glucose; 9002-89-5/Polyvinyl Alcohol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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