| Polyvinyl alcohol and amino acids as substitutes for bovine serum albumin in culture media for mouse preimplantation embryos. | |
| | |
MedLine Citation:
|
PMID: 9286737 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effect of replacing bovine serum albumin (BSA) in a simple defined medium (KSOM) with polyvinyl alcohol (PVA) and/or amino acids on the percentages of mouse zygotes that develop to at least the blastocyst stage and that hatch at least partially or completely is reported. Blastocysts could form when BSA was replaced with only PVA, but at a moderately reduced rate; however, partial hatching, and hence complete hatching, were severely impaired when BSA was replaced with only PVA. The substitution of BSA with amino acids alone resulted in a high rate of blastocyst formation and moderate impairment of hatching. The addition of PVA to BSA-free KSOM supplemented with amino acids had no extra effect. BSA had significant effects when added to BSA-free KSOM supplemented with amino acids. The BSA caused a significant increase in the rate of partial hatching, and may even have had a small effect on the rate of blastocyst formation. The results also showed that glucose, at a high concentration of 5.56 mM, does not inhibit the development of mouse zygotes to hatched blastocysts when cultured in KSOM supplemented with amino acids. |
| | |
Authors:
|
J D Biggers; M C Summers; L K McGinnis |
Related Documents
:
|
11540747 - Effects of microgravity on the binding of acetylsalicylic acid by rhizobium leguminosar... 3417847 - Drug and fatty acid effects on serum thyroid hormone binding. 19594407 - Serum albumin complexation of acetylsalicylic acid metabolites. 8624497 - An unknown hydrolase activity of human serum albumin: beta-lactamase activity. 3137037 - Dialysis fluids and local host resistance in patients on continuous ambulatory peritone... 19226157 - Raising the ceiling of diastereoselectivity in hydrogen transfer on acyclic radicals. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Human reproduction update Volume: 3 ISSN: 1355-4786 ISO Abbreviation: Hum. Reprod. Update Publication Date: 1997 Mar-Apr |
Date Detail:
|
Created Date: 1997-10-09 Completed Date: 1997-10-09 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 9507614 Medline TA: Hum Reprod Update Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 125-35 Citation Subset: IM |
Affiliation:
|
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
pharmacology* Animals Blastocyst / physiology* Culture Media* Culture Techniques Embryonic Development* Female Glucose / pharmacology Mice Polyvinyl Alcohol / pharmacology* Pregnancy Serum Albumin, Bovine / pharmacology* Zygote / growth & development |
| Grant Support | |
ID/Acronym/Agency:
|
UO1-HD21988/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Culture Media; 0/Serum Albumin, Bovine; 50-99-7/Glucose; 9002-89-5/Polyvinyl Alcohol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Human endometriosis-derived permanent cell line (FbEM-1): establishment and characterization.
Next Document: Peptide growth factors and preimplantation development.