Document Detail

Polyunsaturated fatty acids modify mouse hippocampal neuronal excitability during excitotoxic or convulsant stimulation.
MedLine Citation:
PMID:  10536218     Owner:  NLM     Status:  MEDLINE    
The n-3 polyunsaturated fatty acids (PUFAs) reduce cardiac membrane excitability and prevent cardiac arrhythmias in animals and probably in humans. In this study, we assessed the effects of n-3 PUFAs on membrane excitability in mouse hippocampal neurons with both whole-cell current and voltage-clamp methods. Extracellular application of 20 microM eicosapentaenoic acid (EPA, C20:5n-3) significantly reduced the frequency of electrical-evoked action potentials in CA1 neurons of hippocampal slices from 3.8+/-0.7 Hz of control to 2.1+/-0.5 Hz. In addition, EPA significantly hyperpolarized the resting membrane potential and raised the stimulatory threshold of action potentials in CA1 neurons. Another n-3 PUFA, docosahexaenoic acid (DHA, C22:6n-3), had effects on membrane excitability similar to those of EPA. In contrast, EPA ethyl ester, oleic acid (OA, C18:n-9), and stearic acid (SA, C18:0) did not alter the membrane excitability in CA1 neurons. Bath application of pentylenetetrazole (PTZ) or glutamate reduced the stimulatory threshold and increased the frequency of action potentials of hippocampal neurons. EPA restored PTZ- or glutamate-enhanced neuronal excitability to the control level. EPA also suppressed glutamate-activated inward currents. Furthermore, EPA and DHA significantly inhibited the frequency of action potentials without effecting the stimulatory threshold of CA3 neurons. These data demonstrate that n-3 PUFAs modify neuronal membrane excitability under control and drug-stimulated conditions. The sensitivity to these effects of PUFAs varies from neurons of different hippocampal regions.
Y Xiao; X Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research     Volume:  846     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-12-10     Completed Date:  1999-12-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  112-21     Citation Subset:  IM    
The Charles A. Dana Research Institute and The Harvard-Thorndike Laboratory, Cardiovascular Division, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
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MeSH Terms
Action Potentials / drug effects
Docosahexaenoic Acids / pharmacology
Eicosapentaenoic Acid / analogs & derivatives,  pharmacology
Epilepsy / chemically induced,  drug therapy*
Fatty Acids, Unsaturated / pharmacology*
Glutamic Acid / pharmacology
Hippocampus / cytology*
Neurons / drug effects*,  physiology*
Oleic Acid / pharmacology
Patch-Clamp Techniques
Platelet Aggregation Inhibitors / pharmacology
Stearic Acids / pharmacology
Reg. No./Substance:
0/Convulsants; 0/Fatty Acids, Unsaturated; 0/Neurotoxins; 0/Platelet Aggregation Inhibitors; 0/Stearic Acids; 112-80-1/Oleic Acid; 1553-41-9/Eicosapentaenoic Acid; 25167-62-8/Docosahexaenoic Acids; 54-95-5/Pentylenetetrazole; 56-86-0/Glutamic Acid; 57-11-4/stearic acid; 73310-10-8/eicosapentaenoic acid ethyl ester

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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