Document Detail

Polyunsaturated fatty acid pattern in liver and erythrocyte phospholipids from obese patients.
MedLine Citation:
PMID:  17228028     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Our aim was to study the fatty acid (FA) composition of liver phospholipids and its relation to that in erythrocyte membranes from patients with obese nonalcoholic fatty liver disease (NAFLD), as an indication of lipid metabolism alterations leading to steatosis. RESEARCH METHODS AND PROCEDURES: Eight control subjects who underwent antireflux surgery and 12 obese patients with NAFLD who underwent subtotal gastrectomy with a gastro-jejunal anastomosis in Roux-en-Y were studied. The oxidative stress status of patients was assessed by serum F2-isoprostanes levels (gas chromatography/negative ion chemical ionization tandem mass spectrometry). Analysis of FA composition of liver and erythrocyte phospholipids was carried out by gas-liquid chromatography. RESULTS: Patients with NAFLD showed serum F2-isoprostanes levels 84% higher than controls. Compared with controls, liver phospholipids from obese patients exhibited significantly 1) lower levels of 20:4n-6, 22:5n-3, 22:6n-3 [docosahexaenoic acid (DHA)], total long-chain polyunsaturated FA (LCPUFA), and total n-3 LCPUFA, 2) higher 22:5n-6 [docosapentaenoic acid (DPAn-6)] levels and n-6/n-3 LCPUFA ratios, and 3) comparable levels of n-6 LCPUFA. Levels of DHA and DPAn-6 in liver were positively correlated with those in erythrocytes (r = 0.77 and r = 0.90, respectively; p < 0.0001), whereas DHA and DPAn-6 showed a negative association in both tissues (r = -0.79, p < 0.0001 and r = -0.58, p < 0.01, respectively), associated with lower DHA/DPAn-6 ratios. DISCUSSION: Obese patients with NAFLD showed marked alterations in the polyunsaturated fatty acid pattern of the liver. These changes are significantly correlated with those found in erythrocytes, thus suggesting that erythrocyte FA composition could be a reliable indicator of derangements in liver lipid metabolism in obese patients.
Alejandra Elizondo; Julia Araya; Ramón Rodrigo; Jaime Poniachik; Attila Csendes; Fernando Maluenda; Juan C Díaz; Cinzia Signorini; Cristiana Sgherri; Mario Comporti; Luis A Videla
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Obesity (Silver Spring, Md.)     Volume:  15     ISSN:  1930-7381     ISO Abbreviation:  Obesity (Silver Spring)     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-17     Completed Date:  2007-03-20     Revised Date:  2008-01-21    
Medline Journal Info:
Nlm Unique ID:  101264860     Medline TA:  Obesity (Silver Spring)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  24-31     Citation Subset:  IM    
Department of Nutrition, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
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MeSH Terms
Case-Control Studies
Erythrocytes / chemistry,  metabolism
F2-Isoprostanes / analysis,  blood
Fatty Acids, Unsaturated / analysis*
Fatty Liver / etiology,  metabolism*
Liver* / chemistry,  metabolism
Middle Aged
Obesity / complications,  metabolism*
Oxidative Stress
Phospholipids / chemistry*,  metabolism
Reg. No./Substance:
0/F2-Isoprostanes; 0/Fatty Acids, Unsaturated; 0/Phospholipids

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