| Polyunsaturated fatty acid biosynthesis from [1-14C]20:3 n-6 acid in rat cultured Sertoli cells. Linoleic acid effect. | |
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MedLine Citation:
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PMID: 11906823 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Primary culture is a suitable system to study lipid metabolism and polyunsaturated fatty acid biosynthesis. Sertoli cell-enriched preparations were used to determine the fatty acid composition after 5 and 7 days in culture (serum free) as well as the uptake and metabolism of [1-14C]eicosa-8,11,14-trienoic acid. The addition of unlabeled linoleic acid (0.2 and 2.0 microg/ml) was also evaluated. Fatty acid methyl esters derived from cellular lipids were analyzed by gas liquid chromatography and radiochromatography. After 5 days in culture, cells had significantly less 18:2, 20:4, 22:5 and 24:5 and more 18:3, 20:3, 22:4 and 24:4 n-6 fatty acids than non-cultured cells. On day 7, an additional increment in 22:4 n-6 and a decrease in linoleic, gamma-linoleic and 24:4 n-6 fatty acids were observed. The presence of linoleic acid (low dose) produced a significant decrease in saturated and monounsaturated acids and an increase in 18:2, 20:4 and 22:5 n-6 fatty acids. At a high concentration almost all fatty acids belonging to 18:2 n-6 increased significantly. The drop in 20:4 n-6/20:3 n-6 ratio was considered as an indirect evidence of a Delta 5 desaturase activity depression. This assumption was corroborated by studying the transformation of [1-14C]eicosa-8,11,14-trienoic acid into 20:4, 22:4, 22:5, 24:4 and 24:5 n-6 fatty acids. We conclude that Sertoli cells after 7 days in culture evidenced changes in the fatty acid profile similar to those described under fat deprivation. The addition of linoleic acid reverted this pattern and indicated that the Delta 5 desaturase activity is a limiting step in the polyunsaturated fatty acid biosynthesis. |
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Authors:
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Graciela E Hurtado de Catalfo; I Nelva T de Gomez Dumm |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The international journal of biochemistry & cell biology Volume: 34 ISSN: 1357-2725 ISO Abbreviation: Int. J. Biochem. Cell Biol. Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-03-21 Completed Date: 2002-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9508482 Medline TA: Int J Biochem Cell Biol Country: England |
Other Details:
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Languages: eng Pagination: 525-32 Citation Subset: IM |
Affiliation:
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Facultad de Ciencias Médicas, Instituto de Investigaciones Bioquímicas de La Plata-INIBIOLP, Calles 60 y 120, CC 455, 1900 La Plata, Argentina. gehurtado@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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8,11,14-Eicosatrienoic Acid
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metabolism Animals Cells, Cultured Culture Media, Serum-Free Fatty Acids, Omega-6 Fatty Acids, Unsaturated / biosynthesis*, metabolism* Linoleic Acid / administration & dosage, metabolism* Male Rats Rats, Wistar Sertoli Cells / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Serum-Free; 0/Fatty Acids, Omega-6; 0/Fatty Acids, Unsaturated; 2197-37-7/Linoleic Acid; 7324-41-6/8,11,14-Eicosatrienoic Acid |
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