Document Detail


Polystyrene nanoparticle trafficking across MDCK-II.
MedLine Citation:
PMID:  21310266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polystyrene nanoparticles (PNP) cross rat alveolar epithelial cell monolayers via non-endocytic transcellular pathways. To evaluate epithelial cell type-specificity of PNP trafficking, we studied PNP flux across Madin Darby canine kidney cell II monolayers (MDCK-II). The effects of calcium chelation (EGTA), energy depletion (sodium azide (NaN(3)) or decreased temperature), and endocytosis inhibitors methyl-β-cyclodextrin (MBC), monodansylcadaverine and dynasore were determined. Amidine-modified PNP cross MDCK-II 500 times faster than carboxylate-modified PNP. PNP flux did not increase in the presence of EGTA. PNP flux at 4 °C and after treatment with NaN(3) decreased 75% and 80%, respectively. MBC exposure did not decrease PNP flux, whereas dansylcadaverine- or dynasore-treated MDCK-II exhibited ∼80% decreases in PNP flux. Confocal laser scanning microscopy revealed intracellular colocalization of PNP with clathrin heavy chain. These data indicate that PNP translocation across MDCK-II (1) occurs via clathrin-mediated endocytosis and (2) is dependent on PNP physicochemical properties. We conclude that uptake/trafficking of nanoparticles (NPs) into/across epithelia depends both on properties of the NPs and on the specific epithelial cell type.
Authors:
Farnoosh Fazlollahi; Susanne Angelow; Nazanin R Yacobi; Ronald Marchelletta; Alan S L Yu; Sarah F Hamm-Alvarez; Zea Borok; Kwang-Jin Kim; Edward D Crandall
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-26
Journal Detail:
Title:  Nanomedicine : nanotechnology, biology, and medicine     Volume:  7     ISSN:  1549-9642     ISO Abbreviation:  Nanomedicine     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-23     Completed Date:  2012-02-03     Revised Date:  2013-11-12    
Medline Journal Info:
Nlm Unique ID:  101233142     Medline TA:  Nanomedicine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  588-94     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Will Rogers Institute Pulmonary Research Center, University of Southern California, Los Angeles, California 90033, USA. fazlolla@usc.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Membrane Permeability / drug effects*
Clathrin / chemistry,  metabolism
Dogs
Dynamins / metabolism
Egtazic Acid / pharmacology
Endocytosis* / drug effects
Epithelial Cells / drug effects,  metabolism*
Nanoparticles / chemistry*
Polystyrenes / chemistry*,  metabolism*
Rats
Sodium Azide / pharmacology
Surface Properties
Grant Support
ID/Acronym/Agency:
DK062283/DK/NIDDK NIH HHS; ES017034/ES/NIEHS NIH HHS; ES018782/ES/NIEHS NIH HHS; EY011386/EY/NEI NIH HHS; EY017923/EY/NEI NIH HHS; HL038621/HL/NHLBI NIH HHS; HL038658/HL/NHLBI NIH HHS; HL062569/HL/NHLBI NIH HHS; HL089445/HL/NHLBI NIH HHS; R01 DK062283-08/DK/NIDDK NIH HHS; R01 ES017034/ES/NIEHS NIH HHS; R01 ES017034-03/ES/NIEHS NIH HHS; R01 ES017034-04/ES/NIEHS NIH HHS; R01 EY011386-14/EY/NEI NIH HHS; R01 EY017293-05/EY/NEI NIH HHS; R01 HL038621-23/HL/NHLBI NIH HHS; R01 HL038658-15/HL/NHLBI NIH HHS; R01 HL062569/HL/NHLBI NIH HHS; R01 HL062569-09/HL/NHLBI NIH HHS; R01 HL089445/HL/NHLBI NIH HHS; R01 HL089445-04/HL/NHLBI NIH HHS; R37 HL062569/HL/NHLBI NIH HHS; RC2 ES018782-02/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Clathrin; 0/Polystyrenes; 26628-22-8/Sodium Azide; 67-42-5/Egtazic Acid; EC 3.6.5.5/Dynamins
Comments/Corrections

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