| Polystyrene nanoparticle trafficking across MDCK-II. | |
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MedLine Citation:
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PMID: 21310266 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polystyrene nanoparticles (PNP) cross rat alveolar epithelial cell monolayers via non-endocytic transcellular pathways. To evaluate epithelial cell type-specificity of PNP trafficking, we studied PNP flux across Madin Darby canine kidney cell II monolayers (MDCK-II). The effects of calcium chelation (EGTA), energy depletion (sodium azide (NaN(3)) or decreased temperature), and endocytosis inhibitors methyl-β-cyclodextrin (MBC), monodansylcadaverine and dynasore were determined. Amidine-modified PNP cross MDCK-II 500 times faster than carboxylate-modified PNP. PNP flux did not increase in the presence of EGTA. PNP flux at 4 °C and after treatment with NaN(3) decreased 75% and 80%, respectively. MBC exposure did not decrease PNP flux, whereas dansylcadaverine- or dynasore-treated MDCK-II exhibited ∼80% decreases in PNP flux. Confocal laser scanning microscopy revealed intracellular colocalization of PNP with clathrin heavy chain. These data indicate that PNP translocation across MDCK-II (1) occurs via clathrin-mediated endocytosis and (2) is dependent on PNP physicochemical properties. We conclude that uptake/trafficking of nanoparticles (NPs) into/across epithelia depends both on properties of the NPs and on the specific epithelial cell type. |
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Authors:
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Farnoosh Fazlollahi; Susanne Angelow; Nazanin R Yacobi; Ronald Marchelletta; Alan S L Yu; Sarah F Hamm-Alvarez; Zea Borok; Kwang-Jin Kim; Edward D Crandall |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-02-26 |
Journal Detail:
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Title: Nanomedicine : nanotechnology, biology, and medicine Volume: 7 ISSN: 1549-9642 ISO Abbreviation: Nanomedicine Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-09-23 Completed Date: 2012-02-03 Revised Date: 2013-02-08 |
Medline Journal Info:
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Nlm Unique ID: 101233142 Medline TA: Nanomedicine Country: United States |
Other Details:
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Languages: eng Pagination: 588-94 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Will Rogers Institute Pulmonary Research Center, University of Southern California, Los Angeles, California 90033, USA. fazlolla@usc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Membrane Permeability / drug effects* Clathrin / chemistry, metabolism Dogs Dynamins / metabolism Egtazic Acid / pharmacology Endocytosis* / drug effects Epithelial Cells / drug effects, metabolism* Nanoparticles / chemistry* Polystyrenes / chemistry*, metabolism* Rats Sodium Azide / pharmacology Surface Properties |
| Grant Support | |
ID/Acronym/Agency:
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DK062283/DK/NIDDK NIH HHS; ES017034/ES/NIEHS NIH HHS; ES018782/ES/NIEHS NIH HHS; EY011386/EY/NEI NIH HHS; EY017923/EY/NEI NIH HHS; HL038621/HL/NHLBI NIH HHS; HL038658/HL/NHLBI NIH HHS; HL062569/HL/NHLBI NIH HHS; HL089445/HL/NHLBI NIH HHS; R01 DK062283-08/DK/NIDDK NIH HHS; R01 ES017034/ES/NIEHS NIH HHS; R01 ES017034-03/ES/NIEHS NIH HHS; R01 ES017034-04/ES/NIEHS NIH HHS; R01 EY011386-14/EY/NEI NIH HHS; R01 EY017293-05/EY/NEI NIH HHS; R01 HL038621-23/HL/NHLBI NIH HHS; R01 HL038658-15/HL/NHLBI NIH HHS; R01 HL062569/HL/NHLBI NIH HHS; R01 HL062569-09/HL/NHLBI NIH HHS; R01 HL089445/HL/NHLBI NIH HHS; R01 HL089445-04/HL/NHLBI NIH HHS; RC2 ES018782-02/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Clathrin; 0/Polystyrenes; 26628-22-8/Sodium Azide; 67-42-5/Egtazic Acid; EC 3.6.5.5/Dynamins |
| Comments/Corrections | |
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