Document Detail

Polysaccharides from fresh Viscum album L. berry extract and their interaction with Viscum album agglutinin I.
MedLine Citation:
PMID:  10965423     Owner:  NLM     Status:  MEDLINE    
A special microfiltrated colloidal preparation from fresh Viscum album L. berries was investigated concerning the occurrence and structural features of polymeric carbohydrates. A crude polysaccharide fraction was isolated from lectin-, tannin- and protein-depleted microfiltrates. Further fractionation by exchange chromatography revealed a neutral fraction (average molecular weight 30 kDa) and three acidic fractions (average molecular weights 1300 kDa). Structural analysis of the respective polysaccharide fractions by quantitative and qualitative determination of the sugar composition and linkage analysis indicated that all acidic fractions contained an acidic arabinogalactan with a rhamnose-galactoronic acid backbone and highly branched arabinose-galactose side chains attached by the rhamnose residues to the backbone. The neutral fraction consisted of a neutral arabinogalactan beside minor amounts (about 10%) of a low substituted xyloglucan. Further studies on interaction between the 1340 kDa acidic rhamno-arabinogalactans II and III and mistletoe lectin Viscum album agglutinin I (VAA I) revealed binding capacities between these compounds, while the neutral polymers interacted significantly less with VAA I. Only partial binding of VAA I was observed by incubation of the lectin with polysaccharide II. Similar interactions of polysaccharide fraction III with VAA I was measured in a BIACRORE biosensore system. Using the hemagglutination test, increased agglutination of erythrocytes was observed when mistletoes lectin I and the respective polysaccharide fractions were present together in the assay. All these data indicate clearly strong interaction between VAA I and Viscum polysaccharides.
U Edlund; A Hensel; D Fröse; U Pfüller; A Scheffler
Related Documents :
1588843 - Bacteroides and staphylococcus glycocalyx: chemical analysis, and the effects on chemil...
19371033 - Quantitative prediction of cell wall polysaccharide composition in grape (vitis vinifer...
18258213 - Functional domains of the fatty acid transport proteins: studies using protein chimeras.
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Arzneimittel-Forschung     Volume:  50     ISSN:  0004-4172     ISO Abbreviation:  Arzneimittelforschung     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-09-21     Completed Date:  2000-09-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372660     Medline TA:  Arzneimittelforschung     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  645-51     Citation Subset:  IM    
Carl Gustav Carus-Institut, Niefern/Oschelbronn, Friedrich-Alexander Universität Erlangen-Nürnberg, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chromatography, Gas
Chromatography, High Pressure Liquid
Hemagglutination / drug effects
Middle Aged
Mistletoe / chemistry*
Molecular Weight
Plant Extracts / chemistry
Plant Lectins
Plant Preparations*
Plant Proteins*
Plants, Medicinal*
Polysaccharides / chemistry,  pharmacology*
Ribosome Inactivating Proteins
Ribosome Inactivating Proteins, Type 2
Spectrophotometry, Ultraviolet
Toxins, Biological / chemistry,  pharmacology*
Reg. No./Substance:
0/Lectins; 0/Plant Extracts; 0/Plant Lectins; 0/Plant Preparations; 0/Plant Proteins; 0/Polysaccharides; 0/Ribosome Inactivating Proteins, Type 2; 0/Toxins, Biological; 0/VAA-I protein, Viscum album; 0/ribosome inactivating protein, Viscum; EC Inactivating Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Determination of trimebutine and desmethyl-trimebutine in human plasma by HPLC.
Next Document:  Synthesis and antibacterial activity of new arylamido derivatives of 6 beta-aminopenicillanic, 7 bet...