| Polysaccharides from fresh Viscum album L. berry extract and their interaction with Viscum album agglutinin I. | |
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MedLine Citation:
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PMID: 10965423 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A special microfiltrated colloidal preparation from fresh Viscum album L. berries was investigated concerning the occurrence and structural features of polymeric carbohydrates. A crude polysaccharide fraction was isolated from lectin-, tannin- and protein-depleted microfiltrates. Further fractionation by exchange chromatography revealed a neutral fraction (average molecular weight 30 kDa) and three acidic fractions (average molecular weights 1300 kDa). Structural analysis of the respective polysaccharide fractions by quantitative and qualitative determination of the sugar composition and linkage analysis indicated that all acidic fractions contained an acidic arabinogalactan with a rhamnose-galactoronic acid backbone and highly branched arabinose-galactose side chains attached by the rhamnose residues to the backbone. The neutral fraction consisted of a neutral arabinogalactan beside minor amounts (about 10%) of a low substituted xyloglucan. Further studies on interaction between the 1340 kDa acidic rhamno-arabinogalactans II and III and mistletoe lectin Viscum album agglutinin I (VAA I) revealed binding capacities between these compounds, while the neutral polymers interacted significantly less with VAA I. Only partial binding of VAA I was observed by incubation of the lectin with polysaccharide II. Similar interactions of polysaccharide fraction III with VAA I was measured in a BIACRORE biosensore system. Using the hemagglutination test, increased agglutination of erythrocytes was observed when mistletoes lectin I and the respective polysaccharide fractions were present together in the assay. All these data indicate clearly strong interaction between VAA I and Viscum polysaccharides. |
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Authors:
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U Edlund; A Hensel; D Fröse; U Pfüller; A Scheffler |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Arzneimittel-Forschung Volume: 50 ISSN: 0004-4172 ISO Abbreviation: Arzneimittelforschung Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-09-21 Completed Date: 2000-09-21 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372660 Medline TA: Arzneimittelforschung Country: GERMANY |
Other Details:
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Languages: eng Pagination: 645-51 Citation Subset: IM |
Affiliation:
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Carl Gustav Carus-Institut, Niefern/Oschelbronn, Friedrich-Alexander Universität Erlangen-Nürnberg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Chromatography, Gas Chromatography, High Pressure Liquid Hemagglutination / drug effects Humans Lectins Male Methylation Middle Aged Mistletoe / chemistry* Molecular Weight Plant Extracts / chemistry Plant Lectins Plant Preparations* Plant Proteins* Plants, Medicinal* Polysaccharides / chemistry, pharmacology* Ribosome Inactivating Proteins Ribosome Inactivating Proteins, Type 2 Spectrophotometry, Ultraviolet Toxins, Biological / chemistry, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Lectins; 0/Plant Extracts; 0/Plant Lectins; 0/Plant Preparations; 0/Plant Proteins; 0/Polysaccharides; 0/Ribosome Inactivating Proteins, Type 2; 0/Toxins, Biological; 0/VAA-I protein, Viscum album; 0/ribosome inactivating protein, Viscum; EC 3.2.2.22/Ribosome Inactivating Proteins |
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