Document Detail

Polysaccharide synthesis as a carbon dissipation mechanism in metabolically uncoupled Xanthomonas campestris cells.
MedLine Citation:
PMID:  11472799     Owner:  NLM     Status:  MEDLINE    
The utilization of xanthan metabolism as an excess carbon dissipation path in Xanthomonas campestris cells under sub-lethal acid stress was studied. To highlight growth limitation during metabolic uncoupling due to acid toxicity a antibiotic was added. The simultaneous addition of enoxacin and acetic acid showed that the xanthan production per unit of biomass raises with increasing concentrations of enoxacin, which seems to indicate that when the cell is prevented from growing it finds a path to convey the extra carbon. In parallel, although the effect of acetic acid is not very significant, its presence appears to increase xanthan. This tendency seems to be accentuated with increasing concentrations of enoxacin. In fact, in presence of 0.15 mM of acetic acid, 2.88 and 5.76 microM of antibiotic produces xanthan/biomass yields of 8.13 and 9.82 g g(-1) which drop to below half those values (3.55 g g(-1)) when enoxacin is removed. When enoxacin was kept constant, xanthan/biomass yields showed small increments with the increase of acetic acid. Thus, with 1.44, 2.88 and 4.32 microM enoxacin concentrations, the addition of organic acid produces a 6--8% stimulation of xanthan.
M E Esgalhado; A T Caldeira; J C Roseiro; A N Emery
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biotechnology     Volume:  89     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-07-26     Completed Date:  2001-09-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  55-63     Citation Subset:  IM    
School of Chemical Engineering, University of Birmingham, P.O. Box 363, Birmingham B15 2TT, UK.
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MeSH Terms
Carbon / metabolism*
Polysaccharides / biosynthesis*
Polysaccharides, Bacterial / biosynthesis
Proton-Translocating ATPases / metabolism
Xanthomonas campestris / cytology,  enzymology,  metabolism*
Reg. No./Substance:
0/Polysaccharides; 0/Polysaccharides, Bacterial; 11138-66-2/xanthan gum; 7440-44-0/Carbon; EC ATPases

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