| Polysaccharide fractions of Caesalpinia ferrea pods: Potential anti-inflammatory usage. | |
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MedLine Citation:
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PMID: 22178173 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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ETHNOPHARMACOLOGICAL RELEVANCE: Caesalpinia ferrea (Caesalpinioideae), known as pau-ferro or juca, has been used in the traditional medicine in North and Northeast of Brazil in inflammatory disorder, among others. Thus, experimental evaluation of the anti-inflammatory activity of extracts and fraction polysaccharides of Caesalpinia ferrea pods, and correlation with its anti-inflammatory activity and popular use is important. MATERIALS AND METHODS: Total polysaccharides (TPL) were applied to ion exchange chromatography and eluted stepwise. Paw edema was induced s.c. by λ-carrageenan, dextran, histamine, serotonin, compound 48/80, bradykinin, prostaglandin E(2) (PGE(2)) or l-arginine and analyzed by plethysmometry and protein leakage by spectrophotometry. Peritonitis was induced i.p. by carrageenan or N-formyl-methionyl-leucyl-phenylalanine (fMLP) and analyzed 4h later for leukocyte migration and protein leakage. Animals were treated i.v. with TPL or polysaccharide fractions (0.01, 0.1, 1mg/kg) 30min before stimuli in both models. Toxicity (variation of body/organ mass and hematological/biochemical parameters) was evaluated after the seven-day treatment with the most active polysaccharide fraction (1mg/kg; i.v.). RESULTS: Chromatography of TPL (2.8% yield) provided three major polysaccharide fractions (FI, FII, FIII). At 1mg/kg, TPL inhibited the paw edema induced by carrageenan (60%) and FIII (fraction presenting high carbohydrate and low protein content) inhibited the inflammatory parameters in the paw edema induced by the following stimuli: carrageenan (70%), dextran (53%), histamine (65%), serotonin (62%), bradykinin (60%), PGE(2) (63%), nitric oxide (61%) and compound 48/80 (36%). Additionally, FIII at 1mg/kg inhibited the carrageenan-induced edema in animals with intact mast cells, but only the late phase of those with degranulated mast cells elicited by compound 48/80. Moreover, FIII inhibited cell migration and protein leakage in the model of peritonitis elicited by carrageenan (88%) and fMLP (64%), being well tolerated by animals. CONCLUSIONS: Extracts and polysaccharide fractions of Caesalpinia ferrea pods exhibit potent anti-inflammatory activity via negative modulation of histamine, serotonin, bradykinin, PGE(2) and NO released in the carrageenan-induced edema, showing involvement of mast cells. FIII could be interfering not only in the vascular, but also in cellular inflammatory events, revealing to be an important active component of traditionally prepared remedies used to treat inflammatory states. |
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Authors:
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Lívia de Paulo Pereira; Racquel Oliveira da Silva; Pedro Henrique de Souza Ferreira Bringel; Kaira Emanuella Sales da Silva; Ana Maria Sampaio Assreuy; Maria Gonçalves Pereira |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-13 |
Journal Detail:
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Title: Journal of ethnopharmacology Volume: - ISSN: 1872-7573 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7903310 Medline TA: J Ethnopharmacol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ireland Ltd. |
Affiliation:
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Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, 60714-242 Fortaleza, CE, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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