Document Detail

Polysaccharide degradation by Fenton reaction--or peroxidase-generated hydroxyl radicals in isolated plant cell walls.
MedLine Citation:
PMID:  12165299     Owner:  NLM     Status:  MEDLINE    
The formation of hydroxyl radicals (OH*) by peroxidase was confirmed by EPR spectroscopy using ethanol/alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone as a spin-trapping system specific of OH*. The effect of OH*, generated either non-enzymatically with the Fenton reaction (H(2)O(2) + Fe(2+)) or with horseradish peroxidase in the presence of O(2) and NADH, on cell walls isolated from maize (Zea mays) coleoptiles or soybean (Glycine max) hypocotyls was investigated. OH* produced by these reactions attack polysaccharides in the wall, demonstrated by the release of a heterogeneous mixture of polymeric breakdown products into the incubation medium. The peroxidase-catalyzed degradation of cell-wall polysaccharides can be inhibited by KCN and superoxide radical (O(2)*) or OH* scavengers. These data support the hypothesis that OH*, produced by cell-wall peroxidases in vivo, act as wall-loosening agents in plant extension growth.
Carmen Schweikert; Anja Liszkay; Peter Schopfer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytochemistry     Volume:  61     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-08-07     Completed Date:  2002-12-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  31-5     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Elsevier Science Ltd.
Albert-Ludwigs-Universität Freiburg, Institut für Biologie II, Schänzlestr 1, D-79104 Freiburg, Germany.
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MeSH Terms
Cell Wall / metabolism*
Electron Spin Resonance Spectroscopy
Horseradish Peroxidase / metabolism*
Hydrogen Peroxide / metabolism*
Hydroxides / metabolism
Hydroxyl Radical / metabolism*
Iron / metabolism*
Oxidative Stress
Polysaccharides / metabolism*
Soybeans / metabolism
Time Factors
Zea mays / metabolism
Reg. No./Substance:
0/Fenton's reagent; 0/Hydroxides; 0/Polysaccharides; 3352-57-6/Hydroxyl Radical; 7439-89-6/Iron; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.-/Horseradish Peroxidase

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